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目的探讨端粒酶活性对SGC7901胃癌细胞活力的影响。方法 SGC7901细胞分组培养,其中对照组不施加处理因素,其余各组给予端粒酶抑制剂叠氮脱氧胸苷(AZT)即AZT-4、AZT-8及AZT-16组,分别给予AZT 4、8及16 mmol/L,24、48及72 h后以甲基噻唑基四唑(MTT)法检测各组细胞活力,根据MTT实验结果检测AZT抑制SGC7901细胞活力最明显时各组细胞端粒酶活性。结果 AZT可抑制SGC7901细胞活力,呈时间依赖性及剂量依赖性。72 h后AZT-4、AZT-8及AZT-16组端粒酶活性下调率分别为(28.1±4.3)%、(45.4±3.6)%、(63.7±5.1)%,呈明显的剂量依赖性。结论端粒酶活性增强是SGC7901胃癌细胞永生化的重要机制之一。
Objective To investigate the effect of telomerase activity on the viability of SGC7901 gastric cancer cells. Methods SGC7901 cells were cultured in groups of which no treatment was given in the control group and the rest were given AZT-AZT-4, AZT-8 and AZT-16, telomerase inhibitor AZT-4, Cell viability was measured at 8, 16 mmol / L, 24,48, and 72 h by methylthiazolyl tetrazolium (MTT) assay. MTT assay showed that AZT inhibited cell viability of SGC7901 cells active. Results AZT inhibited the viability of SGC7901 cells in a time-and dose-dependent manner. The down-regulation rates of telomerase activity in AZT-4, AZT-8 and AZT-16 groups after 72 h were (28.1 ± 4.3)%, (45.4 ± 3.6)% and (63.7 ± 5.1)% . Conclusion The enhancement of telomerase activity is one of the important mechanisms of immortalization of gastric cancer cell line SGC7901.