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本研究以新颖的极端粗茎水稻材料R404及细茎品种日本晴为亲本杂交构建的F2分离群体(152个单株)为作图群体,对控制水稻茎秆粗、茎壁厚以及穗颈粗3个茎粗度相关性状的QTL进行定位分析。通过QTL Ici Mapping分子标记作图软件分析,在水稻3、8、11以及12号染色体上分别检测到7个QTLs,其中茎粗和穗颈粗的2个QTL q SDM8.1和qRDM8.1可能为同一基因位点调控,茎粗和茎壁厚的2个QTL qSDM3.1及q SWT3.1也定位在相近的位置,这2个染色体位置可能存在控制茎粗的一因多效的基因。qSWT3.1(15.39%)、qRDM3.1(44.66%)和q SDM8.1(19.16%)在其所控制的相应性状(茎粗,茎壁厚和穗颈部粗)中的贡献率是最大,是主效QTL。除了定位于3号染色体的QTLs外,其他与粗茎性状相关的QTLs均为新发现的QTLs。本研究结果为粗茎QTL的进一步精细定位奠定了基础,也为粗茎QTL应用于水稻抗倒伏品种的培育及种质创新提供重要的基因来源,对保障国家粮食安全具有重要的意义。
In this study, F2 segregating population (152 individuals), which was constructed from the novel extremely rough stem R404 and the fine stem variety Nipponbare as parents, was used as a mapping population to control the thickness of the stem, the thickness of the stem, QTLs for traits related to stem diameter were analyzed. Seven QTLs were detected on rice chromosomes 3, 8, 11 and 12 by QTL Ici Mapping molecular marker mapping software. Two QTLs q SDM8.1 and qRDM8.1 Two QTLs qSDM3.1 and q SWT3.1 which are regulated by the same gene locus, stem diameter and stem thickness are also located at similar positions, and the two chromosome positions may have a multiplicative gene that controls stem diameter. The contribution rate of qSWT3.1 (15.39%), qRDM3.1 (44.66%) and q SDM8.1 (19.16%) to the corresponding traits controlled by them (stem diameter, stem wall thickness and panicle neck thickness) , Is the main effect QTL. Except for QTLs located on chromosome 3, other QTLs related to tuber traits were newly discovered QTLs. The results of this study laid the foundation for the further fine mapping of the stem QTLs and provided an important gene source for the cultivation and germplasm innovation of the rice stem lodging resistant varieties, which is of great significance to the national food security.