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目的:检测各种脂肪细胞因子在大鼠急性胰腺炎中的表达及其可能意义。方法:40只SD大鼠随机分为4组:正常对照组、假手术组、急性水肿型胰腺炎(AEP)组和急性坏死型胰腺炎(ANP)组,AEP组腹腔注射雨蛙素,ANP组腹腔注射精氨酸,正常对照组不予以任何处理,假手术组给予同等剂量的生理盐水腹腔注射,应用ELISA法测定大鼠血清中抵抗素、瘦素、脂联素、肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β水平的变化,同时检测血清中淀粉酶(AMY),计算胰腺/体重比值,观察胰腺组织病理变化。结果:AEP组和ANP组血清淀粉酶、胰腺/体重比值、胰腺组织病理评分、血清抵抗素、瘦素、TNF-α、IL-1β水平均较对照组和假手术组明显升高,两者之间差异显著(P<0.01);ANP组较AEP组也升高,两者之间有统计学意义(P<0.01或P<0.05);而AEP组中脂联素水平为(3.26±0.68)mg/L,ANP组中为(2.27±0.45)mg/L,与正常对照组和假手术组比较明显降低,且ANP组较AEP组也降低,统计学上差异显著(P<0.01或P<0.05);血清抵抗素水平与TNF-α、IL-1β水平、胰腺组织病理评分呈正相关,相关系数r为0.711、0.794和0.812(P<0.01);血清瘦素水平与TNF-α、IL-1β水平、胰腺组织病理评分呈正相关,相关系数r为0.736、0.812和0.785(P<0.01或P<0.05),而血清脂联素与TNF-α、IL-1β水平、胰腺组织病理评分呈负相关,相关系数r为-0.796、-0.809、-0.788(P<0.01或P<0.05)。结论:脂肪细胞因子与急性胰腺炎发病发展有关,有可能成为预测急性胰腺炎严重性和预后的有用指标之一,其中抵抗素、瘦素的增加可能加重急性胰腺炎病情的进展,而脂联素增加可能起到保护作用,为急性胰腺炎的治疗提供了新的靶点。
Objective: To detect the expression of various adipocytokines in acute pancreatitis of rats and its possible significance. Methods: Forty SD rats were randomly divided into 4 groups: normal control group, sham operation group, acute edematous pancreatitis (AEP) group and acute necrotic pancreatitis (ANP) group. Intraperitoneal injection of arginine, normal control group without any treatment, the sham operation group were given the same dose of saline intraperitoneal injection, ELISA assay of serum resistin, leptin, adiponectin, tumor necrosis factor (TNF) α and interleukin (IL) -1β in serum were measured. Amylase (AMY) in serum was also measured. Pancreatic / body weight ratio was calculated and pathological changes of pancreas were observed. Results: Serum amylase, pancreas / body weight ratio, pancreatic histopathological score, serum resistin, leptin, TNF-α and IL-1β levels in AEP group and ANP group were significantly higher than those in control group and sham operation group (P <0.01 or P <0.01). The level of adiponectin in ANP group was also significantly higher than that in AEP group (P <0.01 or P <0.05), while the level of adiponectin in AEP group was (3.26 ± 0.68 ) mg / L, and (2.27 ± 0.45) mg / L in ANP group, which were significantly lower than those in normal control group and sham operation group, and also lower in ANP group than in AEP group with statistical significance (P <0.01 or P <0.05). Serum resistin level was positively correlated with TNF-α, IL-1β and pancreatic histopathological score (r = 0.711,0.794, 0.812, P <0.01) (P <0.01 or P <0.05). The levels of serum adiponectin, TNF-α and IL-1β, the pathological score of pancreatic tissue were The correlation coefficient r was -0.796, -0.809, -0.788 (P <0.01 or P <0.05). Conclusion: Adipocyte cytokines are related to the pathogenesis of acute pancreatitis, which may be one of the useful indexes to predict the severity and prognosis of acute pancreatitis. The increase of resistin and leptin may aggravate the progression of acute pancreatitis, Elemental increase may play a protective role, providing a new target for the treatment of acute pancreatitis.