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Objective:To studythe effectof matrineon proliferationof mouseskinfibroblastsinducedby platelet-derivedgrowthfactor-BB(PDGF-BB).Methods :MouseskinfibroblastswereobtainedfromnewbornI-CRmiceandpropagatedin vitro.Proliferationof cellwasanalyzedby mitochondrialreductionof tetrazoliumsalt MTT andactualcellcount.Re sults:Matrine(50to500μg/ml)causeddose-dependentreductionof serum-stimu-latedcellgrowth.Growthinhibitionwas totallyreversedafterremovalof the drug.Matrinealso inhibited PDGF-BBinducedcellgrowthdose-dependently.Conclusion:Matrineexhibitspotentanti-proliferationeffecton mouseskinfibroblast.Thiseffectappearsto be mediatedby decreaseof PDGF-inducedgrowth.Theseresultssug-gestthatmatrinemighthavepreventiveandtherapeuticimplicationin skinfibrosis.
Objective: To study the effect of matrineon proliferation of mouseskin fibroblasts induced by platelet-derived growth factor-BB (PDGF-BB). Methods: Mouseskin fibroblastswereobtainedfromnewbornI-CRmice andpropagated in vitro.Proliferationof cellwasanalyzedby mitochondrialreductionof tetrazoliumsalt MTT andactualcellcount.Re sults: Matrine (50to500μg / ml) causeddose- dependentreductionof serum-stimu- latedcellgrowth . Growth Inhibition was totally reversed after removals of the drug. Matrine also inhibited PDGF-BB induced uterine growth dependent-. Conlusion: Matrine inhibition of potent anti-proliferative effect mouseskin fibroblast. Thiseffectappearsto be mediated by decreaseof PDGF-induced growth. The Severity of Preventive and therapeutic Implications in Skin Fibrosis.