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AIM: To explore the effects of peptide S-8300 from shark liver (S-8300) on liver function as well as in regulating immune functions in experimental injury models.METHODS: Mice were administered with different doses of S-8300 for four consecutive days, followed by mice injected with tetrachloromethane (CCl4) on d 3. The activity of ALT, AST, LDH, SOD and contents of MDA and GSH in the mice liver were tested. And mice were treated with Cy (100 mg/kg) at the beginning of the experiment to induce immunosuppression model. The S-8300 groups were treated with S-8300 seven days after the beginning of Cy administration. The effects of S-8300 on the formulation of serum hemolysin and the content of IL-2 in serum in the immunosuppression mice were observed.RESULTS: S-8300 obviously decreased the level of ALT (52.2±11.0 U/dL vs135.9±6.5 U/dL, P＜0.01), AST (67.5±6.9U/dL vs 238.8±8.7 U/dL, P＜0.01), LDH (155.1±46.8 U/dLvs240.4±6.0 U/dL, P＜0.01) & MDA (0.64±0.027 nmol/mg vs 1.06±0.040 nmol/mg, P＜0.01) and increased SOD(24.51±1.01 U/mg vs19.05±0.73 U/mg, P＜0.01) & GSH (24.17±0.91 μg/mg vs 14.93±0.45 μg/mg, P＜0.01)in mice liver damaged by CCl4. S-8300 also markedly improved the formulation of serum hemolysin (0.094±0.005 A540 vs 0.063±0.006 A540, P＜0.01) and increased the level of IL-2(9.74±1.16 pg/mL vs5.81±0.87 pg/mL, P＜0.01) in serum of the immunosuppression mice.CONCLUSION: The results suggested S-8300 has significant hepatoprotective, immunomodulatory and inhibiting of lipid peroxidation activity.