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目的：研究肝细胞癌（ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ，ＨＣＣ）多药耐药（ｍｕｔｉｄｒｕｇ ｒｅｓｉｓｔａｎｃｅ，ＭＤＲ）基因表达蛋白Ｐ－糖蛋白（Ｐ－ｇｌｙｃｏｐｒｏｔｅｉｎ，Ｐ－ｇｐ））与转移能力的关系。方法：用链霉亲合素－生物素酶复合物（ｓｔｒｅｐｔ ａｖｉｄｉｎ－ｂｉｏｔｉｎｃｏｍｐｌｅｘ，ＳＡＢＣ ）免疫组化方法，检测５７例ＨＣＣ标本中Ｐ－ｇｐ，ｎｍ２３／ＮＤＰＫ，ｒａｓ／ｐ２１，Ｌａｍｉｎｉｎ（ＬＮ），Ｅ－ｃａｄ的含量。结果：Ｐ－ｇｐ阳性百分数为 ５２．６％． Ｐ－ｇｐ阳性和阴性表达者ｎｍ２３阳性白分数分别为４３． ３％和 ７７.７％（ Ｘ~２＝７１． Ｐ＜ ０. ０１），ｒａｓ阳性百分数分别为７６.７％和４４.４％（Ｘ~2＝６.２３，ｐ＜０.０５），ＬＮ阳性百分数分别为２３.３％和３３.３％（Ｘ２＝０.７， ｐ＞ ０.０５）， Ｅ-ｃａｄ阳性百分数分别为 ６０％和 ４４.４％（Ｘ~２＝１.３８， Ｐ＞ ０.０５），侵袭转移率分别为 ７０.０％和４０.７％（Ｘ~2＝４.９４， Ｐ＜ ０.０５）。结论：肝细胞癌Ｐ－ｇｐ高表达与癌细胞侵袭能力增强相关；与ｎｍ２３、ｒａｓ癌基因表达改变有关。
Objective: To investigate the relationship between the expression of P-glycoprotein (P-glycoprotein) and the metastatic potential of multidrug resistance (MDR) gene in hepatocellular carcinoma (HCC). METHODS: P-gp, nm23/NDPK, ras/p21, Laminin (LN) were detected in 57 specimens of HCC by strept avidin-biotin complex (SABC) immunohistochemistry. E-cad content. Results: The positive percentage of P-gp was 52.6%. The nm23 positive white fraction of P-gp positive and negative expression were 43. At 3% and 77.7% (X~2=71. P<0.01), the percentage of ras positive was 76.7% and 44.4%, respectively (X~2=6.23, p<0.05). The positive percentage of LN was 23.3% and 33.3% (X2=0.7, p> 0.05), respectively, and the positive percentage of E-cad was 60% and 44.4% (X~2=1. 38, P> 0.05), Invasion and metastasis rates were 70.0% and 40.7%, respectively (X~2=4.94, P<0.05). Conclusion: The high expression of P-gp in hepatocellular carcinoma is related to the enhancement of the invasive ability of cancer cells, and related to the expression of nm23 and ras oncogenes.