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提取、分离了雷公藤 (Tripterygium wilfordii Hook)根皮中总生物碱 ,并对其杀虫活性和作用方式进行了系统的生物测定。结果表明 ,雷公藤总生物碱对 5龄菜青虫有很强的麻醉、拒食和毒杀作用 ;拒食中浓度AFC5 0 为 2 75 .5 3m g/L,毒杀中浓度 L C5 0 为 77.86 m g/L,麻醉中量 ND5 0 为 2 .2 9μg/g;雷公藤总生物碱无触杀和熏蒸作用。试虫中毒症状主要为幼虫表皮出现黑斑 ,形成黑斑部分不能脱去旧表皮 ,最终死亡
The total alkaloids in the root bark of Tripterygium wilfordii Hook were extracted and separated, and the bioassay of the insecticidal activity and mode of action was systematically carried out. The results showed that the total alkaloids of Tripterygium wilfordii had strong anesthetic, antifeedant and toxic effects on Pieris rapae on the 5th instar larvae. The concentrations of antifeedant AFC5 0 were 2 75.53 m g / L and the toxic concentrations of L C5 0 were 77.86 mg / L, the amount of anesthesia ND5 0 was 2. 29μg / g; Tripterygium total alkaloids no contact and fumigation. Symptoms of pest poisoning are mainly the appearance of dark spots on the epidermis of larvae, the formation of dark spots can not take off the old epidermis and eventually die