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为了研究水环境中盐度、碱度对中国林蛙蝌蚪的毒性效应,试验采用单因子静态急性毒性试验法对其进行了研究。结果表明:pH值为7.0~8.5、总碱度为1.41 g/L时,盐度对中国林蛙蝌蚪24,48,72,96 h零致死量(LC0)分别为7.15,5.80,3.43,2.83 g/L,半致死量(LC50)分别为8.21,7.26,5.24,4.08 g/L,全致死量(LC100)分别为9.26,8.80,7.71,5.33 g/L,安全值(SC)为1.93 g/L;在相同pH值下,盐度为0.18 g/L时,碱度对中国林蛙蝌蚪24,48,72,96 h LC50分别为14.91,12.31,10.35,7.68 g/L,LC0分别为8.76,8.51,4.65,3.88 g/L,LC100分别为19.34,16.15,16.07,11.48 g/L,SC值为3.05 g/L。因此,认为中国林蛙蝌蚪长期生存的盐度上限为2.00 g/L,碱度上限为2.50 g/L。
In order to study the toxic effects of salinity and alkalinity on tadpoles of Rana chensinensis in water environment, the single factor static acute toxicity test was used to study the toxicity effects. The results showed that the LC0 values of salinity to Tadpole of Rana chensinensis at 24, 48, 72 and 96 h were 7.15, 5.80, 3.43 and 2.83 respectively at pH values of 7.0-8.5 and total alkalinity of 1.41 g / L The LC50 were 8.21, 7.26, 5.24 and 4.08 g / L respectively, the LC100 values were 9.26, 8.80, 7.71 and 5.33 g / L respectively, and the safety value was 1.93 g / L; At the same pH, salinity of 0.18 g / L, the alkalinity of Chinese Rana tadpole 24,48,72,96 h LC50 were 14.91,12.31,10.35,7.68 g / L, LC0 were 8.76, 8.51, 4.65 and 3.88 g / L, respectively. The LC100 values were 19.34, 16.15, 16.07 and 11.48 g / L, respectively. The SC value was 3.05 g / L. Therefore, the upper limit of salinity of long-term survival of Chinese frog tadpoles is 2.00 g / L and alkalinity is 2.50 g / L.