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根据Laxer等克隆的微小隐孢子虫(C.parvum)核酸序列片断(pHCl)设计并合成一对长度为20hp的寡核苷酸引物.应用聚合酶链反应,对隐孢子虫核酸进行扩增,结果扩增出452hP的特异性条带,阴性对照无扩增.结果表明所合成引物有高度特异性.用PCR检测隐抱子虫感染动物模型,36份镜检卵囊阳性小鼠粪便均为阳性,18份镜检阴性小鼠粪便17份阴性,一份阳性.“,”Institute of Parasitology, Sun Yat-sen University of Medical Sciences, Guangzhou, 510089A pair of 20-base primers were designed and synthesized according to the nucleotide sequence fragment ofC. parvum cloned by Laxer. Primers were applied to amplifying C. parvum DNA by polymerase chain reaction andspecific amplification of a 452bp product was demonstrated by ethidium bromide staining of 2% agarose gel electrophorisis. Negative control showed negative results. The rusult indicated that primers had high specific for C.parvum. Animal model infected with C. parvum was detected by PCR. Thirty-six mice faeces samples being positive microscopically were also all positive. One of eighteen samples being negative microscopically was positive.This results suggested that PCR method should be higher sensitivity than the microscopic method.