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目的探讨镉、铅、乐果和对硫磷对未成年雌性小鼠雌激素样联合作用。方法选择断乳后17天龄SPF级雌性昆明小鼠192只,体重(25±2)g,随机分为16个组:空白对照组,氯化镉、醋酸铅、乐果和对硫磷组单独作用12个组,联合作用3个组。给药浓度为氯化镉、醋酸铅、乐果和对硫磷LD50(分别为9.3、140、45和3mg/kg)的1/20、1/50及1/100,给药体积为10ml/kg BW,每天腹腔注射,连续3天。实验第4天,处死小鼠,测量子宫脏器系数、子宫上皮细胞高度及子宫雌激素受体(ER)表达。结果氯化镉1/50LD50、醋酸铅1/50LD50及联合作用1/100LD50剂量组能显著改变子宫脏器系数;氯化镉各剂量组、醋酸铅1/20LD50剂量组和联合作用的1/20LD50、1/50LD50剂量组可引起未成年雌性小鼠子宫上皮细胞高度的显著增加,即引起子宫内膜的明显增生;除氯化镉和乐果的1/20LD50、1/50LD50剂量组其他所有实验组与空白对照比较均能显著增强雌激素受体(ER)的表达。结论镉、铅、乐果和对硫磷对未成年雌性小鼠雌激素样联合作用与单因素作用存在显著差异,且在不同剂量不同指标中表现不尽相同。
Objective To investigate the combined effects of cadmium, lead, dimethoate and parathion on female mice. Methods A total of 192 SPF female Kunming mice aged 17 days after weaning were randomly divided into 16 groups: blank control group, cadmium chloride, lead acetate, dimethoate and parathion alone Effect of 12 groups, the combined effect of three groups. Administration concentrations were 1/20, 1/50 and 1/100 of cadmium chloride, lead acetate, dimethoate and parathion LD50 (9.3, 140, 45 and 3 mg / kg, respectively) at a dose of 10 ml / kg BW, intraperitoneal injection for 3 consecutive days. On the fourth day of the experiment, the mice were sacrificed and the uterine organ coefficient, uterine epithelial cell height and uterine estrogen receptor (ER) expression were measured. Results The cadmium chloride 1 / 50LD50, lead acetate 1 / 50LD50 and combined effect 1 / 100LD50 dose group could significantly change the uterine organ coefficient; cadmium chloride dose groups, lead acetate 1 / 20LD50 dose group and the combined effect of 1 / 20LD50,1 / 50LD50 dose group can cause a significant increase in the uterine epithelial cells of young female mice, which caused significant proliferation of endometrial; except cadmium chloride and dimethoate 1 / 20LD50,1 / 50LD50 dose group all other experimental groups and Compared with blank control, the expression of estrogen receptor (ER) was significantly increased. Conclusion The combination of cadmium, lead, dimethoate and parathion on the estrogen-like activity of the female mice is significantly different from that of the single factor, and their performance varies with different dosages.