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[目的]研究氟对大鼠睾丸组织过氧化氢酶(CAT)、乳酸脱氢酶(LDH)和丙二醛(MDA)水平的影响以及钙对其拮抗作用。[方法]选用健康雄性清洁级SD大鼠80只,随机分为10组:对照组、加钙染氟6个剂量组[NaF 2.1、4.2、8.5、17.0、34.0、68.0 mg/(kg·d);CaCO325、50、50、100、100、150 mg/(kg·d)]和不加钙染氟3个剂量组[NaF2.1、8.5、34.0 mg/(kg·d)],灌胃染毒90 d。染毒结束次日,观察大鼠精子数量、睾丸组织结构的改变,并测定睾丸组织中CAT、LDH和MDA水平。[结果]氟可使大鼠睾丸精子计数下降并导致睾丸组织病理学改变。各组大鼠睾丸组织CAT、LDH和MDA含量组间比较,差异有统计学意义(F值分别为5.168、2.248、3.168,P值分别为<0.001、0.044、0.007)。高剂量染氟组CAT[(27.86±4.04)U/mg prot]较对照组[(44.85±2.81)U/mg prot]降低、LDH[(38 358.49±2 649.23)U/g prot]较对照组[(54 357.66±1 903.90)U/g prot]降低、MDA[(6.82±0.79)nmol/mg prot]较对照组[(3.59±1.11)nmol/mg prot]升高。在相同染氟浓度下,适量加钙组较不加钙组CAT水平升高、MDA水平降低。[结论]氟引起睾丸组织的损害可能与氧化应激作用有关,适量的钙可以拮抗氟诱导的自由基水平增高所造成的氧化损伤作用。
[Objective] To investigate the effects of fluoride on the levels of catalase (CAT), lactate dehydrogenase (LDH) and malondialdehyde (MDA) in rat testis and the antagonistic effect of calcium on it. [Methods] Eighty healthy male SD rats were randomly divided into 10 groups: the control group and the calcium-added fluoride 6-dose group [NaF 2.1, 4.2, 8.5, 17.0, 34.0, 68.0 mg / (kg · d ); CaCO325,50,50,100,100,150 mg / (kg · d)], and no dose of calcium fluoride [NaF2.1,8.5,34.0 mg / (kg · d)], Toxicity 90 d. The day after the end of the exposure, the number of spermatozoa and the changes of the testicular tissue structure were observed. The levels of CAT, LDH and MDA in the testis were also determined. [Results] Fluorine decreased the testicular sperm count in rats and led to pathological changes of testicular tissue. The contents of CAT, LDH and MDA in testis of rats in each group were significantly different (F = 5.168,2.248,3.168, P = 0.001,0.044,0.007 respectively). Compared with the control group ([(44.85 ± 2.81) U / mg prot], LDH [(38 358.49 ± 2 649.23) U / g prot] [(54 357.66 ± 1 903.90) U / g prot] and MDA [(6.82 ± 0.79) nmol / mg prot] were higher than those in control group [(3.59 ± 1.11) nmol / mg prot] Under the same fluoride concentration, the level of CAT and the level of MDA decreased in the moderate calcium group compared with the non-calcium group. [Conclusion] The damage of testicular tissue induced by fluoride may be related to the oxidative stress. The proper amount of calcium can antagonize the oxidative damage caused by the increase of fluoride-induced free radicals.