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目的:利用大鼠复发性口疮(ROU)动物模型,探讨T淋巴细胞、氧自由基、微循环与ROU发病机制的关系。方法:用大鼠口腔黏膜匀浆组织加弗氏免疫佐剂注射法建立大鼠ROU模型,应用流式细胞仪检测实验前后CD4+细胞、CD8细胞的变化;分别用黄嘌呤氧化酶法和化学法检测实验前后超氧化物歧化酶(superoxide dismutase,SOD)、谷+光甘肽过氧化物酶(glutathine peroxidease,GSH-Px)和过氧化脂质分解产物(malondialdehyde,MDA)的改变;分别用生化分析法和微量毛细管法检测实验前后纤维蛋白原和红细胞压积的变化,NEX-2型板式粘度计测定实验前后血浆粘度。结果:①实验前后CD4/CD8比值下降,有显著性差异(p<0.05);②实验前后SOD下降,++GSH-Px下降,MDA升高(p<0.01);③实验前后纤维蛋白原、红细胞压积、血浆粘度均升高(p<0.01)。结论:①ROU的发生与T淋巴细胞失调有关;②证实了动物口腔溃疡黏膜的损伤系氧自由基产生所致;③成模后的大鼠存在微循环障碍。
OBJECTIVE: To investigate the relationship between T lymphocytes, oxygen free radicals, microcirculation and pathogenesis of ROU in rats with recurrent aphthous ulcer (ROU). Methods: ROU model was established by oral mucosal homogenate and Freund’s adjuvant injection in rats. Flow cytometry was used to detect the changes of CD4 + cells and CD8 cells before and after the experiment. Xanthine oxidase method and chemical method The changes of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) before and after the test were detected. The changes of fibrinogen and hematocrit before and after the experiment were detected by the method of analysis and micro-capillaries. The viscosity of the plasma was measured by the NEX-2 plate viscometer before and after the experiment. Before and after the experiment, the ratio of CD4 / CD8 decreased significantly (p <0.05); ② Before and after the experiment, SOD decreased, ++ GSH-Px decreased, MDA increased (p <0.01); ③ Before and after the experiment, fibrinogen, Hematocrit, plasma viscosity were increased (p <0.01). Conclusion: ① The occurrence of LUU is related to the imbalance of T lymphocytes; ② It is confirmed that the damage of oral ulcer mucosa is caused by oxygen free radicals; ③ There is microcirculation disturbance in rats after molding.