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检测了３株分化不同的人胃癌细胞ＭＫＮ－４５、ＭＫＮ－２８、ＳＧＣ－７９０１在体外培养过程中产生免疫抑制因子的体外免疫抑制作用，分化差的ＭＫＮ－４５及源于转移性ＳＧＣ－７９０１胃癌细胞株体外培养过程中产生免疫抑制因子对正常人外周血单个核细胞（ＰＢＭＣ）产生ＩＬ－２水平及其ＩＬ－２Ｒ表达抑制作用较强，且显著高于高分化ＭＫＮ－２８细胞株产生免疫抑制因子的作用；而正常ＲＰ－ＭＩ１６４０细胞培养液无免疫抑制作用。这一研究结果表明：胃癌细胞本身可自行分泌免疫抑制物质或因子，同时提示胃癌患者机体免疫功能低下不仅与体内免疫抑制细胞有关，而且与胃癌细胞本身产生免疫抑制因子不可分割，对指导今后胃癌免疫治疗具有重要指导意义
The in vitro immunosuppressive effects of three differently differentiated human gastric cancer cells, MKN-45, MKN-28, and SGC-7901, produced immunosuppressive factors during in vitro culture. The poorly differentiated MKN-45 and metastatic SGC-7901 were detected. Immunosuppressive factors produced during the in vitro culture of gastric cancer cell lines have a strong inhibitory effect on the production of IL-2 and the expression of IL-2R in peripheral blood mononuclear cells (PBMCs) of normal individuals, and are significantly higher than those produced by well-differentiated MKN-28 cell lines. The role of immunosuppressive factors; and normal RP-MI1640 cell culture medium without immunosuppressive effect. The results of this study indicate that gastric cancer cells can secrete immunosuppressive substances or factors by themselves, and at the same time, they suggest that the immune function of gastric cancer patients is not only related to immunosuppressive cells in vivo, but also inseparable with the immunosuppressive factors produced by gastric cancer cells themselves. Immunotherapy has important guiding significance