目的验证HBV表面抗原大蛋白(LHBs)与人胰腺突触角蛋白6(STX6)在细胞内是否存在相互作用,为进一步研究HBV影响糖、脂代谢机制奠定研究基础。方法构建真核表达质粒pACT-STX6,采用哺乳动物双杂交技术验证LHBs与STX6之间的相互作用。结果实验组和各阴性对照组相对荧光素酶活性值均存在差异,且均具有显著统计学意义(P<0.01)。结论 LHBs与人胰腺STX6在胰腺细胞内存在相互作用。 Objective To verify whether there is interaction between HBV surface antigen large protein (LHBs) and human pancreatic synapse keratin 6 (STX6) in cells, and lay the foundation for further study on the mechanism of HBV affecting glucose and lipid metabolism. Methods The eukaryotic expression plasmid pACT-STX6 was constructed and the mammalian two-hybrid technique was used to verify the interaction between LHBs and STX6. Results The relative luciferase activity of experimental group and each negative control group were different, and both had significant statistical significance (P <0.01). Conclusion LHBs and human pancreas STX6 exist in the interaction of pancreatic cells.