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在肯尼亚Machakos、Kitui和Baringo区选择28例无并发症的内脏利什曼病人为观察对象,其中18例为男性,10例为女性,平均年龄为11岁。18例病人治疗后随访3个月,其中16例继续随访了12个月。同时按年龄性别在研究区内选择20名无病史的健康人为对照组。从每个观察对象中抽取10ml静脉血,与2ml含10％ EDTA的4.5％葡聚糖混和。在37℃下沉淀45分钟。把含有大量白细胞的血浆铺在3ml histopaque 1077上,用histop-
In Machakos, Kitui and Baringo districts of Kenya, 28 patients with visceral leishmaniasis without complications were selected as observational subjects, of whom 18 were male and 10 were female, with an average age of 11 years. Eighteen patients were followed up for 3 months after treatment, 16 of them were followed up for 12 months. At the same time, 20 healthy people without history of disease were selected as the control group in the study area by age and sex. Ten ml of venous blood was drawn from each observation and mixed with 2 ml of 4.5% dextran containing 10% EDTA. Precipitate 45 minutes at 37 ° C. The plasma containing a large amount of white blood cells was spread on 3 ml histopaque 1077 and treated with histop-