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目的:观察脑缺血再灌注损伤大鼠急性期和恢复早期的半胱氨酰白三烯受体(CysLT2R)蛋白及其基因mRNA的表达,探讨CysLT2R在各组之间表达的差异,观察黄芩苷和栀子苷对其表达的影响。方法:56只雄性SD大鼠,分为假手术组、急性期和恢复早期模型对照组、栀子苷和黄芩苷配伍组,醒脑静组。各组预防给药4d后,采用线栓法复制大鼠脑缺血再灌注模型,缺血24h后和72h后,处死大鼠,剥离海马组织,观察海马组织病理改变,采用wester-blot法Real Time-PCR技术检测脑缺血再灌注损伤急性期和恢复早期大鼠海马组织中CysLT2R蛋白和mRNA的表达情况。结果:与假手术组比较,栀子苷和黄芩苷配伍(9mg+21mg)可改善脑缺血急性期及恢复早期组织病理变化。脑缺血急性期和恢复早期模型组大鼠海马组织CysLT2R蛋白表达均明显升高。栀子苷和黄芩苷配伍组大鼠海马组织CysLT2R的蛋白表达明显降低;海马CysLT2RmRNA表达在模型组急性期和恢复早期样本中增加,在栀子苷和黄芩苷配伍组中表达减少。结论:栀子苷和黄芩苷配伍可对脑缺血急性期和恢复期大鼠CysLT2R蛋白和mRNA表达升高产生一定的抑制作用。
OBJECTIVE: To observe the expression of CysLT2R protein and its gene mRNA in the acute phase and early recovery of cerebral ischemia-reperfusion injury in rats, and to explore the difference of CysLT2R expression between the groups, Effects of Glycosides and Geniposide on Their Expression. Methods: Fifty-six male Sprague-Dawley rats were divided into sham operation group, acute phase and early recovery model control group, geniposide and baicalin compatibility group and Xingnaojing group. After 4 days of administration, rats were sacrificed and the rats were sacrificed after 24 hours and 72 hours after ischemia, respectively. The rats were sacrificed and the hippocampus was dissected. The pathological changes in the hippocampus were observed by wester-blot method Real Time-PCR was used to detect the expression of CysLT2R protein and mRNA in the hippocampus of the acute phase of cerebral ischemia-reperfusion injury and early recovery. Results: Compared with the sham-operation group, the combination of geniposide and baicalin (9mg + 21mg) could improve the histopathological changes during the acute phase of cerebral ischemia and early recovery. The expression of CysLT2R protein in hippocampus of acute cerebral ischemia group and early recovery model group were significantly increased. The protein expression of CysLT2R in hippocampus of rats in combination with geniposide and baicalin significantly decreased. The expression of CysLT2R mRNA in hippocampus increased in acute phase and early recovery of model group, and decreased in the compatibility group of geniposide and baicalin. Conclusion: The combination of geniposide and baicalin can inhibit the expression of CysLT2R protein and mRNA in acute and convalescent phase of cerebral ischemia in rats.