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肌苷(Inosine)能提高细胞内多种酶的活性,参与能量代谢和蛋白质合成。有证据说明肌苷有明显的强心作用,并对哇巴因引起的犬心律不齐有对抗作用。乌头碱(Aconitine)是一种膜去极化剂,常作为筛选抗心律失常药物的模型。本工作观察了乌头碱对豚鼠室间隔心肌细胞自发节律动作电位的异常影响,以及肌苷对这种异常影响的恢复作用,在单细胞水平为肌苷对心律失常的治疗作用提供了依据。 实验用健康豚鼠心肌室间隔,台氏液灌流(95％O_2+5％CO_2,34℃)。将微电极插入心肌细胞,进行细胞内记录。肌苷和乌头碱分别加入台氏液中,作用于心肌标本。标本中保留窦房结,故心肌细胞存在自发的节律性动作电位,可稳定2～3小时。记录这种自发动作电位的周期(T),50％和100％复极化动作电位时限(APD50和APD100),以此说明乌
Inosine increases the activity of many enzymes in the cell and is involved in energy metabolism and protein synthesis. There is evidence that inosine has a pronounced cardiotonic effect and antagonism of ouabain-induced canine arrhythmia. Aconitine is a membrane depolarizer and is often used as a model for screening antiarrhythmic drugs. This study observed the abnormal effect of aconitine on spontaneous rhythmic action potentials of guinea pig ventricular septal cardiac myocytes and the effect of inosine on this abnormal effect, and provided basis for the therapeutic effect of inosine on arrhythmia at single cell level. Experimental use of healthy guinea pig ventricular septum, Tyrode’s solution perfusion (95% O 2 +5% CO_2, 34 ℃). Insert the microelectrode into the cardiomyocytes for intracellular recording. Inosine and aconitine were added to Tyrode’s solution, acting on the myocardial specimens. Sinus node specimens retained, so there is spontaneous myocardial cell rhythmic action potential can be stable 2 to 3 hours. The period (T), the 50% and 100% repolarization action potentials (APD50 and APD100) of this spontaneous action potential were recorded to illustrate