复元胶囊对晚期膝骨关节炎大鼠软骨中Ⅱ型胶原及基质金属蛋白酶1和3的影响

来源 :中国新药与临床杂志 | 被引量 : 0次 | 上传用户:skal1125
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的观察复元胶囊对实验性大鼠晚期膝骨关节炎软骨组织中基质金属蛋白酶1(MMP-1)、MMP-3及Ⅱ型胶原的影响,探讨其治疗骨关节炎的作用机制。方法成年雄性SD大鼠60只,随机分为六组,每组10只。除正常组外,其余各组均采用Hulth法建立晚期膝骨关节炎模型。手术后第2周开始,抗骨增生胶囊组予抗骨增生胶囊557 mg·kg-1·d-1,复元胶囊低、中、高剂量组分别给予复元胶囊371.65、743.30、2 229.90 mg·kg-1·d-1,正常组和模型组给予等体积生理盐水,每日灌胃1次,持续12周。12周后评价各组大鼠关节肿胀情况,并取大鼠胫骨平台的软骨组织。按Pelletier-JP标准评价关节软骨大体情况,并通过HE染色进行Mankin’s评分。采用免疫组织化学法和Western blot法检测各组关节软骨中MMP-1、MMP-3及Ⅱ型胶原的蛋白表达量。结果与正常组相比,模型组大鼠关节肿胀评分、PelletierJP标准和Mankin’s评分均升高(P<0.05或P<0.01),而各给药组关节肿胀评分、Pelletier-JP标准和Mankin’s评分均低于模型组(P<0.05或P<0.01)。复元胶囊中、高剂量组Mankin’s评分低于抗骨增生胶囊组(P<0.05或P<0.01)。与正常组相比,模型组软骨组织中MMP-1、MMP-3表达量明显升高,Ⅱ型胶原表达量明显降低(P<0.01)。各给药组MMP-1、MMP-3表达量低于模型组,Ⅱ型胶原表达量高于模型组(P<0.05或P<0.01)。复元胶囊高剂量组MMP-1、MMP-3的表达量低于抗骨增生胶囊组和复元胶囊低、中剂量组,Ⅱ型胶原表达量高于抗骨增生胶囊组和复元胶囊低、中剂量组(P<0.05或P<0.01)。结论复元胶囊能通过抑制MMPs的表达,减少胶原的降解,达到对晚期骨关节炎的防治作用。 Objective To observe the effect of Fuyuan Capsule on the expressions of matrix metalloproteinase-1 (MMP-1), MMP-3 and collagen Ⅱ in the cartilage tissue of advanced knee osteoarthritis in rats and to explore its mechanism of action in treating osteoarthritis. Methods Sixty adult male Sprague-Dawley rats were randomly divided into six groups of 10 rats. In addition to the normal group, the rest of the groups were established using Hulth method of advanced knee osteoarthritis model. At the second week after operation, the anti-bone-augmentation capsule group was given 557 mg · kg-1 · d-1 anti-bone-augmentation capsules and the low, middle and high dose groups were given Fuyuan capsules 371.65, 743.2 and 229.90 mg · kg- -1 · d-1, normal group and model group were given equal volume of saline, gavage once daily for 12 weeks. Twelve weeks later, the swollen joints of the rats in each group were evaluated, and the cartilage of the tibial plateau in rats was taken. The general condition of articular cartilage was evaluated according to the Pelletier-JP standard, and Mankin’s score was determined by HE staining. Immunohistochemistry and Western blot were used to detect the protein expression of MMP-1, MMP-3 and type Ⅱ collagen in articular cartilage of each group. Results Compared with the normal group, the joint swelling index, PelletierJP standard and Mankin’s score in the model group were significantly increased (P <0.05 or P <0.01), while the joint swelling score, Pelletier-JP standard and Mankin’s score Lower than model group (P <0.05 or P <0.01). Mankin’s score in Fuyuan capsule group was lower than that in Kangaojianjian Capsule group (P <0.05 or P <0.01). Compared with normal group, the expression of MMP-1 and MMP-3 in cartilage tissue of model group was significantly increased and the expression of type Ⅱ collagen was significantly decreased (P <0.01). The expression of MMP-1 and MMP-3 in each administration group was lower than that in model group, and the expression of type II collagen was higher than that in model group (P <0.05 or P <0.01). The expression of MMP-1 and MMP-3 in Fuyuan capsule high-dose group was lower than that in anti-bone-augmentation capsule group and Fuyuan capsule low- and middle-dose group, and the expression of typeⅡcollagen was higher than that in anti-bone-augmentation capsule group and Fuyuan capsule Group (P <0.05 or P <0.01). Conclusion Fuyuan capsule can inhibit the expression of MMPs and reduce the degradation of collagen to achieve the prevention and treatment of advanced osteoarthritis.
其他文献
采用溶胶-凝胶法制备二氧化钛溶胶,将其浸渍在硅胶上,合成了负载型纳米二氧化钛材料.以ICPAES为检测手段,系统地研究了负载型纳米二氧化钛材料对稀土元素La,Y,Yb和Eu的吸附性
荧光法研究痕量的生物大分子与各种小分子、离子的相互作用具有灵敏度高、方法简单、快速等特点.本文用EB,即溴化乙锭,为荧光探针研究各种不同茶叶多糖和多酚类化合物对DNA的
注意缺陷多动障碍(ADHD)是一种以多动、冲动和注意缺陷为核心症状的儿童期慢性疾病,其公认有效的治疗方法包括药物治疗和行为干预。本文就儿童ADHD的治疗药物,包括中枢兴奋剂
目的比较2种硝苯地平缓释片在健康人体的药动学和生物等效性。方法40例男性健康志愿者进行随机双交叉试验,分别单剂量和多剂量口服硝苯地平缓释片受试制剂和参比制剂,采用LC-
通过不同的实验条件合成假性紫罗兰酮,探讨了催化剂用量、反应物配比、反应温度和反应时间等因素对反应产率的影响,通过实验最终得到提高合成假性紫罗兰酮收率的较佳反应条件
以纳米SiO2为吸附材料,研究了其对Cr(Ⅲ)和Cr(Ⅵ)的吸附特性,结果表明,在一定pH条件下,纳米SiO2对Cr(Ⅲ)可大量吸附,而对Cr(Ⅵ)基本不吸附,从而达到分离富集的目的.在选定的
分子对接是实现合理药物设计的重要方法之一.利用Chemscore作为能量函数,以及将一种新的优化算法一部落粒子群算法作为搜索算法,得到一种新的对接程序:Tribe-PSO Dock;利用Tr
会议
由于飞行职业对身体的要求,飞行员每日摄入的热量和营养素量大。退休以后,运动量减少,能量需要减少,长期养成的高糖、高脂肪饮食习惯易造成肥胖。而肥胖者易患糖尿病、高血
荧光免疫检测是一类利用荧光分子为标记进行免疫学检测的重要生物分析方法.在此方法中,抗原或抗体的荧光标记的方式和数目将极大的影响检测方法的灵敏度.由于在抗体上直接标
会议
共递送抗癌药多柔比星和基因治疗肿瘤可以产生协同治疗作用,提高治疗效果,降低毒性以及克服多柔比星引起的多药耐药性。共递送抗癌药和基因的纳米载体近年成为国内外研究的热