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目的探讨 CD_(15),CD44v6和nm23H1的 mRNA 表达与大肠癌临床病理参数和预后的关系及相关性。方法应用原位杂交检测90例大肠癌中 CD_(15),CD44v6和nm23H1的 mRNA 表达及20例原发灶和转移灶大肠癌配对标本中 CD_(15) mRNA 表达,并结合53例5a 以上随访资料分析。结果90例大肠癌中 CD_(15),C1N4v6和nm23H1的 mRNA 阳性表达分别为76例(84.4%),62例(68.9%)和60例(66.7%),CD_(15)和 CD44v6的 mRNA 高表达及 nm23H1 mRNA 低表达与大肠癌 Duke’s 分期、浆膜浸润、淋巴结转移、肝脏转移均呈正相关,大肠癌中 CD_(15) mRNA 表达与 CD44v6 mRNA 表达呈正相关,与 nm23H1 mRNA 表达呈负相关,20例原发灶和转移灶大肠癌配对标本中,CD_(15) mRNA 表达水平一致。结论大肠癌的侵袭转移与相关基因协同作用密切相关,在大肠癌的侵袭转移中 CD_(15),CD44v6和 nm23H1的 mRNA 表达具有正、负调节的协同作用可能,CD_(15) mRNA 可作为一个新的准确预测大肠癌侵袭转移潜能,并客观评估患者预后的生物学指标。
Objective To investigate the relationship between the mRNA expression of CD_ (15), CD44v6 and nm23H1 and the clinicopathological parameters and prognosis of colorectal cancer. Methods The mRNA expression of CD_ (15), CD44v6 and nm23H1 in 90 cases of colorectal cancer and 20 cases of colorectal cancer matched samples of primary and metastatic colorectal cancer were detected by in situ hybridization. 53 patients with more than 5a follow-up ANALYSE information. Results The mRNA expressions of CD_ (15), C1N4v6 and nm23H1 in 90 cases of colorectal cancer were 76 (84.4%), 62 (68.9%) and 60 (66.7% The expression of nm23H1 mRNA and the low expression of nm23H1 mRNA were positively correlated with Duke’s staging, serosal invasion, lymph node metastasis and liver metastasis. Colorectal cancer CD_ (15) mRNA expression was positively correlated with CD44v6 mRNA expression, but negatively correlated with nm23H1 mRNA expression Cases of primary and metastatic colorectal cancer paired specimens, CD_ (15) mRNA expression levels are consistent. Conclusion The invasion and metastasis of colorectal carcinoma is closely related to the synergistic effect of related genes. The mRNA expression of CD_ (15), CD44v6 and nm23H1 may have positive and negative synergistic effects in the invasion and metastasis of colorectal cancer. CD_ (15) mRNA may serve as a New accurate prediction of invasion and metastasis of colorectal cancer, and objective assessment of prognosis in patients with biological indicators.