1924年马氏等用运动试验时心率与血压作为判定心脏功能的标准,1941年马氏等应用运动试验直接描记心电图方法,作为诊断冠心病的手段,1963年马氏等提出了标准化的双倍二级梯运动试验方法。经过成千上万次的临床实践,虽然认为在冠心病高发病地区,马氏双倍二级梯运动试验价廉安全简易,皆可作为筛选方法,但是运动负荷量小,不足以激发出冠状血管循环不足的表现,故可导致假阴性,而在低发病地区则可出现较多的假阳性,并且单纯以性别、年龄及体重作为运动量标准 Markov in 1924 and other exercise test heart rate and blood pressure as a criterion for determining cardiac function, such as Markov 1941 exercise test described the direct ECG method, as a means of diagnosis of coronary heart disease, such as Markov in 1963 proposed a standardized double Two ladder test method. After thousands of times of clinical practice, though it is considered that in the high prevalence of coronary heart disease, Markov double second-step exercise test is cheap and safe and can be used as a screening method, but the exercise load is small, not enough to stimulate the coronary Vascular insufficiency of the performance, it can lead to false negative, while in areas with low incidence of false positives can occur more, and simply based on gender, age and weight as exercise standards