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目的比较三氯乙烯(TCE)致敏豚鼠和未致敏豚鼠皮肤和血清中IL-17表达水平,探讨TCE药疹样皮炎新的发病机制。方法将豚鼠随机分为空白对照组,溶剂(橄榄油)对照组,TCE处理组。根据豚鼠最大值试验方法(Guinea Pig Maximization Test,GPMT)处理豚鼠。按照《化学品毒性鉴定技术规范》的评分标准对动物的皮肤反应进行评分,评分≥1的判为致敏。在末次激发后24 h,72 h,1周和2周分四批采血和皮肤组织,用ELISA试剂盒测定血清中IL-17的含量;将皮肤组织制成蜡块,采用Elivison二步法免疫组织化学法检测其IL-17的表达情况。结果根据皮肤反应评分判断致敏阳性,TCE处理组致敏率为70%。TCE处理组血清中IL-17的水平及皮肤组织免疫组化评分明显高于溶剂对照组,且差异有统计学意义(P<0.05);TCE处理组的不同时间段相比,IL-17的表达差异有统计学意义(P<0.05);在TCE处理组72h和1周两个时点,TCE致敏鼠IL-17的表达比相应的未致敏鼠高,差异有统计学意义(P<0.05)。结论 TCE可以诱导豚鼠致敏,IL-17在豚鼠致敏的过程中有重要意义。
Objective To compare the expression of IL-17 in skin and serum of sensitized guinea pigs and non-sensitized guinea pigs with trichlorethylene (TCE), and to explore the new pathogenesis of TCE-like dermatitis. Methods Guinea pigs were randomly divided into blank control group, solvent (olive oil) control group and TCE treatment group. Guinea pigs were treated according to the Guinea Pig Maximization Test (GPMT). Animal skin reactions were scored according to the scoring criteria of the Technical Specifications for Toxicity Identification of Chemicals and the score ≥1 was judged as sensitization. Four batches of blood and skin tissues were collected at 24 h, 72 h, 1 wk and 2 wk after the last challenge. The content of IL-17 in the serum was determined by ELISA kit. The skin tissue was made into wax blocks, and Elivison two-step immunization Histochemical method was used to detect the expression of IL-17. Results According to the skin reaction score, the sensitization was positive. The sensitization rate of TCE treatment group was 70%. The level of IL-17 in serum of TCE-treated group and the immunohistochemical score of skin tissue were significantly higher than that of solvent-control group (P <0.05). Compared with the control group, the levels of IL-17 The expression of IL-17 in TCE-sensitized mice was higher than that in corresponding non-sensitized mice at 72 h and 1 week after TCE treatment (P <0.05), and the difference was statistically significant (P <0.05) <0.05). Conclusion TCE can induce guinea pig sensitization, IL-17 is important in the process of guinea pig sensitization.