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目的:研究疏肝饮及其组分对反复避水应激(RWAS)大鼠内脏痛觉过敏(VHL)的调节作用,并探讨疏肝饮是否通过调节背根神经节(DRG)中瞬时受体电位通道香草酸受体1(TRPV1)和结肠组织中P物质(SP)表达起作用。方法:应用RWAS法复制具有VHL特点的肠易激综合征(IBS)大鼠模型,观察疏肝饮及其组分对结直肠扩张(CRD)引起大鼠腹壁收缩反射(AWR)压力阈值的影响;分别应用免疫组化法和Real-time PCR法检测大鼠结肠组织中SP和DRG中TRPV1的表达变化。结果:模型大鼠引起AWR的压力阈值明显低于正常对照组,疏肝饮可以使模型大鼠引起AWR的压力阈值明显升高;疏肝饮及其组分可以降低模型大鼠结肠组织中高表达的SP和DRG中高表达的TRPV1。结论:疏肝饮可以改善RWAS大鼠的VHL,其作用机制与其调节结肠组织中SP和DRG中TRPV1的表达有关。
Objective: To study the regulatory effect of Shugan Decoction and its components on visceral hyperalgesia (VHL) in rats with recalcitrant water stress (RWAS) and to explore whether Shugan Yin regulates the expression of transient receptor in DRG Potential channel vanilloid receptor 1 (TRPV1) and substance P (SP) expression in colon tissue play a role. Methods: The rat model of irritable bowel syndrome (IBS) with VHL was replicated by RWAS, and the effect of Shugan Decoction and its components on the pressure threshold of abdominal contraction induced by colorectal distension (CRD) in rats was observed The changes of TRPV1 expression in the SP and DRG of the colon were detected by immunohistochemistry and Real-time PCR respectively. Results: The pressure threshold induced by AWR in model rats was significantly lower than that in normal control group. Shugan Yin could significantly increase the pressure threshold induced by AWR in model rats. Shugan Decoction and its components can reduce the expression of overexpression in colon tissue of model rats The SP and DRG are highly expressed in TRPV1. Conclusion: Shugan Decoction can improve VHL in RWAS rats, and its mechanism is related to the regulation of TRPV1 expression in SP and DRG in colonic tissues.