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目的观察舒乐洗剂治疗外阴阴道念珠菌病(VVC)的效果。方法建立体外白念珠菌生物膜模型,采用XTT法检测生物膜抑制率;建立VVC小鼠模型,采用阴道涂片检查和病理组织检测法,比较给药前后炎症状况的变化。结果舒乐洗剂对体外白念珠菌生物膜的形成有明显抑制作用,并成一定的剂量依赖性;能够抑制体外白念珠菌菌落形成,其中原液稀释一倍的效果最为显著;能够明显减少小鼠体内白念珠菌菌落形成,并能够减少炎性细胞向阴道黏膜的浸润。结论舒乐洗剂具有体外抗生物膜形成的作用,并能降低体内小鼠白念珠菌的感染状况,减轻VVC小鼠阴道黏膜炎症,从而达到治疗VVC的作用。
Objective To observe the effect of Shuxe Lotion on vulvovaginal candidiasis (VVC). Methods The Candida albicans biofilm model was established in vitro. The inhibition rate of biofilm was detected by XTT method. VVC mouse model was established. Vaginal smear and pathological examination were used to compare the changes of inflammation before and after administration. Results Shule Lotion could significantly inhibit the formation of Candida albicans biofilm in vitro and in a dose-dependent manner. It could inhibit the formation of Candida albicans colony in vitro. The dilution of the stock solution was the most significant. Candida albicans colony formation in mice, and can reduce the infiltration of inflammatory cells to the vaginal mucosa. Conclusions Shule lotion has the effect of inhibiting the formation of biofilm in vitro, and can reduce the infection of Candida albicans in mice and relieve the inflammation of vaginal mucosa in VVC mice so as to achieve the effect of treating VVC.