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中粒种咖啡幼胚在含Kt2.0毫克/升+2.4-D0.5毫克/升+NAA0.2毫克/升的培养基上能产生疏松、半透明的愈伤组织;愈防组织在Kt1.0毫克/升+GA0.5毫克/升的培养基上分化出大量小子叶型胚状体;刚形成的胚状体团在含Kt2.0毫克/升+活性炭0.5％的培养基上迅速增殖,形成难以计数的大量胚状体。
Medium grain coffee immature embryos in the Kt2.0 mg / L +2.4-D0.5 mg / L + NAA0.2 mg / L medium can produce loose, translucent callus; more anti-tissue in Kt1 .0 mg / L + GA0.5 mg / L medium on the differentiation of a large number of small cotyledon embryoid body; just formed embryoid body containing Kt2.0 mg / L + 0.5% activated carbon rapidly on the medium Proliferate, forming a large number of embryoid body difficult to count.