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目的 :用幽门螺杆菌 (Helicobacterpylori)长期感染蒙古沙土鼠建立胃炎、胃溃疡的动物模型。方法 :蒙古沙土鼠经过禁食、禁水预处理之后 ,采用国际标准菌株H .pyloriATCC 4 35 0 4灌胃 ,在末次灌胃动物后 4 ,8,12 ,2 4 ,4 8,5 2周分别对H .pylori感染组和正常对照组蒙古沙土鼠的胃黏膜进行H .pylori形态学 (涂片和细菌培养 )、H .pylori快速尿素酶检测、H .pylori特异性PCR检测和病理组织学观察 ,并对血清中抗H .pyloriIgG抗体进行了检测。结果 :H .pylori感染组动物以上各项指标均为阳性 ,表明已经感染H .pylori;而正常对照组则以上各项均为阴性。病理组织学观察表明感染组 8周即可见到慢性炎症性病变 ,12周时黏膜层和黏膜下层可见到淋巴滤泡 ,2 4周胃窦部可见到胃溃疡。结论 :H .pyloriATCC 4 35 0 4可以长期定植于蒙古沙土鼠腺胃 ,能够产生与H .pylori感染人胃黏膜相似的病理改变
Objective: To establish a chronic gastritis and gastric ulcer animal model by long-term infection of gerbils with Helicobacter pylori. Methods: The Mongolian gerbils were fed with international standard strain H.pylori ATCC 4 35 0 4 after fasting and water forbidden treatment. After 4, 8, 12, 24, 48, and 52 weeks after the last intragastric administration, The H.pylori morphology (smear and bacterial culture), H.pylori rapid urease assay, H.pylori specific PCR assay and histopathology were performed on H.pylori infection group and normal control group respectively. Observed, and serum anti-Hpylori IgG antibodies were detected. Results: All the above indexes of H.pylori infection group were positive, which indicated that H.pylori had been infected, while those of the normal control group were all negative. Histopathological examination showed that chronic inflammatory lesions were found in the infected group at 8 weeks. Lymphoid follicles were observed in the mucosa and submucosa at 12 weeks and gastric ulcer at 24 weeks. CONCLUSION: H.pylori ATCC 4 35 0 4 can be chronically colonized in the glandular stomach of Mongolian gerbils and produce similar pathological changes as H.pylori-infected human gastric mucosa