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该法快速,简单。将含有游离苯妥英(DPH)的血清直接进样至HPLC 柱中测定,采用内表面反相硅胶柱。游离和结合型DPH 峰的分辨受流动相含异丙醇的百分比、进样血清的体积和样品中蛋白质浓度等诸因素的影响。游离和结合型药物浓度的测定结果与用简便的超滤办法的测定结果相一致。
The method is fast and easy. Serum containing free phenytoin (DPH) was injected directly into the HPLC column using an internal reverse phase silica gel column. The resolution of free and bound DPH peaks is influenced by factors such as the percentage of mobile phase isopropanol, the volume of sample injected and the protein concentration in the sample. The results of the determination of free and combined drug concentrations are in agreement with the results of a simple ultrafiltration assay.