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目的:观察高压氧预适应(HBOP)及低压缺氧预适应(HHP)对实验性家兔大脑中动脉闭塞(MCAO)后梗塞灶体积的影响,探讨HBOP及HHP的神经保护作用。方法:雄性家兔72只随机分为单纯MCAO组、HBOP+MCAO组,HHP+MCAO组,采用改良Bederson法建立家兔MCAO模型,分别于MCAO后1d、3d、10d及20d观察家兔行为学、病理学及脑梗塞灶体积。结果:行为学评分,单纯MCAO组第1天为3.91±0.14,第3d为3.42±0.47,第10d为2.03±0.56,第20d为1.97±0.35;HBOP+MCAO组和HHP+MCAO组的行为学评分则明显改善(P<0.05),HBOP+MCAO组分别为3.58±0.27(1d)、2.85±0.69(3d)、1.63±0.81(10d)和1.55±1.12(20d),HHP+MCAO组分别为3.47±0.46(1d)、2.78±0.7(3d)、1.5±1.02(10d)和1.41±0.82(20d)。HBOP+MCAO组和HHP+MCAO组的脑梗塞灶体积分别于MCAO后3d、10d和20d也小于单纯MCAO组(P<0.05),单纯MCAO组则为(375.6±15.6)mm3(3d)、(257.5±9.1)mm3(10d)和(231.5±13.9)mm3(20d),而HBOP+MCAO组分别为(332.0±17.6)mm3(3d)、(202.2±12.2)mm3(10d)和(195.3±14.3)mm3(20d),HHP+MCAO组分别为(324.2±15.4)mm3(3d)、(208.1±12.1)mm3(10d)和(201.9±9.9)mm3(20d),但HBOP+MCAO组和HHP+MCAO组之间无明显差异。结论:HBOP和HHP能明显改善实验性MCAO家兔行为学,缩小梗塞灶体积,有一定的神经保护作用。
Objective: To observe the effects of HBOP and Hypoxic preconditioning (HHP) on infarct volume in experimental rabbit middle cerebral artery occlusion (MCAO), and to explore the neuroprotective effects of HBOP and HHP. Methods: Seventy-two male rabbits were randomly divided into MCAO group, HBOP + MCAO group and HHP + MCAO group. Rabbit MCAO model was established by modified Bederson method. The rabbits’ behavior was observed on the 1st, 3rd, 10th and 20th day after MCAO , Pathology and cerebral infarction volume. Results: Behavioral score in the MCAO group was 3.91 ± 0.14 on the first day, 3.42 ± 0.47 on the third day, 2.03 ± 0.56 on the 10th day, and 1.97 ± 0.35 on the 20th day in MCAO group. The behavior of the MCAO group and the HOP + MCAO group (P <0.05). HBO + MCAO group were 3.58 ± 0.27 (1d), 2.85 ± 0.69 (3d), 1.63 ± 0.81 (10d) and 1.55 ± 1.12 3.47 ± 0.46 (1d), 2.78 ± 0.7 (3d), 1.5 ± 1.02 (10d) and 1.41 ± 0.82 (20d). The volume of cerebral infarction in HBOP + MCAO group and HHP + MCAO group was also smaller than that of MCAO group at 3d, 10d and 20d after MCAO (375.6 ± 15.6) mm3 (3d) 257.5 ± 9.1 mm3 and 231.5 ± 13.9 mm3 respectively for the HBOP + MCAO group (332.0 ± 17.6) mm3 (3d), (202.2 ± 12.2) mm3 (10d) and (195.3 ± 14.3) ) in the HOP + MCAO group were (324.2 ± 15.4) mm3 (3d), (208.1 ± 12.1) mm3 (10d) and (201.9 ± 9.9) mm3 There was no significant difference between MCAO groups. Conclusion: HBOP and HHP can significantly improve the behavior of experimental MCAO rabbits, reduce infarct size and have certain neuroprotective effect.