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目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)与子痫前期发病的相关性。方法纳入河北省沧州市中心医院2013年1月至2014年11月确诊为子痫前期的患者100例,其中轻度子痫前期的患者51例,重度子痫前期患者49例,同时纳入同期分娩的健康孕妇50例作为对照组。采用Western blotting、反转录酶-聚合酶链反应分子生物学方法检测3组患者胎盘组织中VEGF、MMP-9的表达水平;采用酶联免疫吸附方法检查患者血清及尿液中VEGF、MMP-9的水平。结果轻、重度子痫前期组患者的胎盘组织中VEGF、MMP-9的表达水平均低于正常对照组,且组间相比差异有统计学意义(P<0.05);轻、重度子痫前期组患者血清及尿液中VEGF、MMP-9的水平均低于正常对照组,且组间相比差异有统计学意义(P<0.05)。结论 VEGF、MMP-9参与了子痫前期的病理生理改变,且与病情的发生及轻重程度有密切相关性。
Objective To investigate the correlation between the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in preeclampsia. Methods One hundred patients diagnosed as preeclampsia from January 2013 to November 2014 in Central Hospital of Cangzhou, Hebei Province were enrolled. Among them, 51 cases were mild preeclampsia and 49 cases were severe preeclampsia, Healthy pregnant women 50 cases as the control group. Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) were used to detect the expression of VEGF and MMP-9 in the placenta of the three groups. The levels of VEGF and MMP-9 in the serum and urine of the patients were detected by enzyme-linked immunosorbent assay 9 level. Results The expression levels of VEGF and MMP-9 in placenta of patients with mild and severe preeclampsia were significantly lower than those of normal control group (P <0.05). The levels of VEGF and MMP-9 in placenta of patients with mild and severe preeclampsia were significantly lower than those of normal control group The levels of VEGF and MMP-9 in serum and urine of the patients were lower than those in the normal control group, and there was significant difference between the two groups (P <0.05). Conclusion VEGF and MMP-9 are involved in the pathophysiological changes of preeclampsia and are closely related to the occurrence and the severity of the disease.