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目的研究载银纳米二氧化钛对噬菌体MS2的灭活效果。方法采用悬液定量灭活试验,对载银纳米二氧化钛水溶液对噬菌体MS2的灭活效果进行了观察。结果用40 g/L的硫代硫酸钠溶液作为中和剂对500 mg/L载银纳米二氧化钛消毒剂残留物达不到中和效果,不能满足判定标准要求。用离心法按照8000 rpm离心10 min,可在不损失噬菌体MS2的情况下去除500 mg/L载银纳米二氧化钛对噬菌体的作用,以此替代中和剂法。在自然光下,载银纳米二氧化钛的浓度为50 mg/L作用2 min,其对噬菌体MS2灭活对数值均>4。结论载银纳米二氧化钛在自然光照下对噬菌体MS2的灭活效果较好。
Objective To study the inactivation effect of silver nanoparticles loaded with nano-titania on bacteriophage MS2. Methods Suspension quantitative inactivation test was used to observe the inactivation effect of silver nanoparticles loaded with titanium dioxide on bacteriophage MS2. Results With 40 g / L sodium thiosulfate solution as the neutralizer, 500 mg / L of silver nano-titania disinfectant could not reach the neutralization effect, which could not meet the criteria of judgment. Centrifugation at 8000 rpm centrifugation 10 min, without loss of bacteriophage MS2 500 mg / L silver nanoparticles loaded with titanium dioxide on phage, as an alternative to neutralization method. In natural light, silver nanoparticles loaded with titanium dioxide concentration of 50 mg / L for 2 min, the phagocytosis of MS2 logarithmic values were> 4. Conclusion AgN2 nanoparticles have good inactivation effect on bacteriophage MS2 in natural light.