食用菌的单孢子分离技术,无论在遗传学研究上,还是在育种应用上都十分重要。用于丝状真菌的单孢分离方法甚多,而简便、快速、准确为其基本要求。我们研究试制的DBFL-1型机械式单孢分离器,在诱变育种及杂交育种实验中得到了广泛应用,并取得了较理想的效果。它的主要优点是容易掌握、准确性好。现将其使用方法介绍如下: 1.将分离器固定圈套固在物镜上〔物镜要求为5x～6x〕。 2.揿动软线定刀头处于水平位置后,用软轴线定位器放松定位。 Edible fungus single spore separation technology, both in genetics research, or breeding applications are very important. For filamentous fungi single spore separation method is very simple, fast, accurate as its basic requirements. The DBFL-1 type mechanical single spore separator researched and developed by us has been widely used in mutagenesis breeding and hybrid breeding experiments and achieved satisfactory results. Its main advantage is easy to grasp, good accuracy. Here’s how to use it: 1. Fix the splitter snare on the objective lens (objective requirement is 5x ~ 6x). 2. 软 move the cord fixed blade in the horizontal position, with soft axis positioner to relax positioning.