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雌激素和孕酮对产生前列腺素的影响已有广泛研究,而雌激素对子宫前列腺素合成的确切作用尚无定论。本文是在确定雌二醇对离体大鼠子宫自发活动及其对释放到溶液中PGE和PGF样物质的影响。材料和方法:雌性成年大鼠,重约190～230g。每天阴道涂片确定其激素情况。处死前20～25天,乙醚麻醉下行卵巢切除。按Nei l方法于处死前3天用雌激素治疗,即第一天于12时皮下注射内含0.5μg17-β雌二醇的30%乙醇0.2ml,第二天12时再皮下注射内含1μg或50μg17-β雌二醇的30%乙醇0.2ml。第三天,即在上次注射24小时后棒击头部处死动物,取出子宫放入盛有含糖的克-任氏重碳酸
The effect of estrogen and progesterone on the production of prostaglandins has been extensively studied, and the exact role of estrogen in utero prostaglandin synthesis is inconclusive. This article is to determine the effect of estradiol on isolated rat uterine spontaneous activity and its effect on PGE and PGF-like substances released into solution. MATERIALS AND METHODS: Female adult rats weighing about 190-230 g. Daily vaginal smear to determine the hormone situation. 20 to 25 days before sacrifice, ovariectomy under ether anesthesia. According to Nei l method 3 days before sacrifice with estrogen treatment, namely the first day at 12 o’clock subcutaneous injection of 0.5μg17-beta estradiol in 30% ethanol 0.2ml, the next day and then subcutaneous injection containing 1μg Or 50 μg of 17% β-estradiol in 30% ethanol 0.2 ml. The third day, that is, after the last injection of 24 hours after the head battled the animals killed, remove the uterus into sugar-containing grams - Ren’s bicarbonate