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本文采用硫酸铵盐析、DE-52离子交换层析、Sephadex G-200凝胶过滤及抗SP_1免疫亲和柱层析等方法从胎盘后血分离妊娠特异性β_1民糖蛋白(SP_1)所得SP_1纯化约207倍.纯化的SP_1与德国Behringwerke AG的SP_1抗血清在双向免疫扩散时有沉淀反应;免疫电泳时纯化SP_1移动的位置与β蛋白相当.聚丙烯酰胺凝胶电泳时纯化的SP_1呈现一条主带其分子量为90,000左右.免疫家免所得的抗血清经血浆蛋白吸收后得特异SP_1抗血清在双向免疫扩散时与正常男子或非孕妇女血清均无沉淀反应;与孕血反应形成的沉淀线与德国Behring werke AG的SP_1抗血清所形成的沉淀线相连;交义免疫电泳时呈现一个峰.此抗血清已成功地用于建立酶联免疫法测定SP_1含量
In this paper, SP_1 was isolated from placental blood by the method of ammonium sulfate salinization, DE-52 ion exchange chromatography, Sephadex G-200 gel filtration and anti-SP_1 immunoaffinity column chromatography Purified about 207. The purified SP_1 and the SP 1 antiserum from Behringwerke AG in Germany have a precipitation reaction in two-way immunodiffusion, and the location of the purified SP_1 moves in the same way as the beta protein in the immunoelectrophoresis.A purified SP_1 in polyacrylamide gel electrophoresis shows one The main band of its molecular weight of about 90.000 immune-derived anti-serum obtained after the plasma protein-specific SP 1 antiserum in two-way immunodiffusion no precipitation reaction with normal men or non-pregnant women serum; Line was connected with the precipitation line formed by the SP_1 antiserum from Behring werke AG, Germany. The antisense immunoelectrophoresis showed a peak which was successfully used to establish the enzyme-linked immunosorbent assay