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分别用原位杂交（ＩＳＨ）和过氧化物酶与抗过氧化物酶复合物（ＰＡＰ）法检测了７３例肝细胞癌（ＨＣＣ）患者肝组织切片中的丙型肝炎病毒（ＨＣＶ）ＲＮＡ和ＨＣＶ抗原（ＨＣＶＡｇ）以及乙型肝炎病毒的表面抗原（ＨＢｓＡｇ）和核心抗原（ＨＢｃＡｇ）。结果显示：ＨＣＶＲＮＡ，ＨＣＶＡｇ，ＨＢｓＡｇ和ＨＢｃＡｇ的检出率分别为２６．０％，２８．８％，７５．３％和２６．。％；ＨＣＶ阳性的肝细胞多呈散在型分布，可见有水肿变性并伴周围炎细胞浸润，ＨＣＶ染色颗粒既可见于肝细胞浆也可见于细胞核；ＨＣＶ标志物（ＨＣＶＭ）的检出与ＨＢＶ的感染状况无显著相关。提示：ＨＣＶ可在ＨＣＣ患者的肝细胞浆和核中存在并表达，ＨＣＶ感染可能在我国ＨＣＣ的发生中起一定的病原学作用。
In situ hybridization (ISH) and peroxidase-peroxidase complex (PAP) methods were used to detect hepatitis C virus (HCV) RNA in liver tissue sections from 73 patients with hepatocellular carcinoma (HCC). HCV antigen (HCVAg) and surface antigen (HBsAg) and core antigen (HBcAg) of hepatitis B virus. The results showed that the detection rates of HCVRNA, HCVAg, HBsAg and HBcAg were 26.0%, 28.8%, 75.3% and 26.0%, respectively. . %; HCV positive hepatocytes are mostly scattered type, showing edematous degeneration with peripheral inflammatory cell infiltration, HCV staining particles can be found in both hepatocyte cytoplasm and nucleus; detection of HCV marker (HCVM) and HBV The infection status was not significantly related. Tip: HCV can be present and expressed in the cytoplasm and nucleus of HCC patients. HCV infection may play a role in the pathogenesis of HCC in China.