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目的探讨锰离子(Mn2+)增强功能MRI成像对确定癫发作相关脑区的价值。并进一步确定癫与钙超载的相关性,从而对癫的发病机制和定位进行研究。方法给成年猫肌肉注射戊四氮(PTZ)制作癫疒间模型,观察猫的行为学和脑电图改变;在癫疒间急性发作时和发作后24h进行Mn2+功能MRI成像检查;对信号明显增强的脑区做病理学检查并与对照组比较。结果PTZ致疒间猫脑电图呈阵发性高波幅棘-慢波。癫发作组猫Mn2+功能MRI成像显示大脑皮质明显弥漫性增强,其中额、顶、枕叶脑皮质增强率达34.6%,颞叶皮质增强率达约22.9%,与对照组相比差异有极显著性(均P<0.01)。癫疒间发作后24h组Mn2+功能MRI成像仍显示额、顶叶明显强化。强化区的神经元有明显变性、坏死。结论额、顶叶为PTZ致疒间猫癫疒间发作形成的相关脑区,Mn2+增强功能MRI成像能显示癫疒间发作的部位,并可进一步揭示癫疒间发病机制。
Objective To investigate the value of manganese (Mn2 +) enhanced MRI in determining brain regions associated with epileptic seizures. And further determine the correlation between epilepsy and calcium overload, so as to study the pathogenesis and location of epilepsy. Methods Intramuscular injection of pentylenetetrazol (PTZ) into adult cats was made into epilepsy models to observe the behavioral and electroencephalographic changes of cats. Mn2 + functional MRI imaging was performed at 24 hours after the onset of epileptic seizures. Enhanced brain regions were examined by pathology and compared with the control group. Results PTZ caused by intercostal cat EEG paroxysmal high amplitude spike - slow wave. In the epileptic seizure group, Mn2 + functional MRI showed diffuse enhancement of the cerebral cortex. The enhancement rate of the frontal, parietal and occipital cortex was 34.6% and the temporal cortex enhancement rate was about 22.9%, which was significantly different from the control group Significance (all P <0.01). 24 hours after the onset of epilepsy Mn2 + functional MRI imaging still showed the amount of parietal lobes significantly enhanced. Fortified area neurons have obvious degeneration and necrosis. Conclusions: The parietal and parietal lobes are related brain regions in the PTZ-induced cats epilepsy, and Mn2 + -funced functional MRI can reveal the site of epilepsy and further reveal the pathogenesis of epilepsy.