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目的 建立人成骨肉瘤细胞系。方法 采用原代组织块培养法对成骨肉瘤手术标本进行培养 ,对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶 (LDH)同工酶谱、细胞周期检查、核型分析、异种移植。结果 建立细胞系SOSP 96 0 7,其形态学表现、组织化学染色、LDH同工酶谱等均符合成骨肉瘤的特征。经 1年体外培养 ,已连续传代 12 0次 ,细胞倍增时间 2 9.8h ,细胞周期测定G1期为 48.2 %、G2 期为 2 0 .9%、S期为 30 .9%。染色体具有亚三倍体核型 ,众数为 6 4～ 6 6条。异种移植成瘤率 10 0 % ,无支原体污染。结论 人成骨肉瘤细胞系SOSP 96 0 7可用于对成骨肉瘤的研究。
Objective To establish a human osteosarcoma cell line. Methods Osteoblastoma surgical specimens were cultured with primary tissue culture method. Morphological observation, histochemical staining, isoenzymes of lactate dehydrogenase (LDH) isoforms, cell cycle examination, karyotype analysis, and heterogeneity were performed on surviving cells. transplant. Results The cell line SOSP 96 0 7 was established and its morphological features, histochemical staining, and LDH isoenzyme spectrum were all consistent with the characteristics of osteosarcoma. After 1 year of in vitro culture, it has been serially passaged 12 times, and the cell doubling time was 29.8 hours. The G1 phase of the cell cycle assay was 48.2%, the G2 phase was 20.9%, and the S phase was 30.9%. Chromosomes have a sub-triploid karyotype with a mode number of 64 to 66. Tumor formation rate of xenotransplantation was 100%, no mycoplasma contamination. Conclusion The human osteosarcoma cell line SOSP 96 0 7 can be used for the study of osteosarcoma.