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目的：研究Ｄｏｗｎ’ｓ 综合征动物模型ｔｒｉｓｏｍｙ １６ 结肠神经系发育和先天性巨结肠（ＨＤ） 病变肠管蛋白基因产物９－５（ｐｒｏｔｅｉｎ ｇｅｎｅ ｐｒｏｄｕｃｔ９ ．５ ，ＰＧＰ９－５） 的神经表达。方法：Ｔｒｉｓｏｍｙ １６ 鼠培育；细胞遗传学分析；Ｔｒｉｓｏｍｙ １６ 鼠结肠和ＨＤＰＧＰ９－５ 免疫组织化学。结果：（１）Ｔｒｉｓｏｍｙ １６ 鼠结肠神经系发育异常，肌间神经丛发育迟缓，粘膜下神经丛缺失，结肠末端有５ ｍｍ 的无神经节区，但结肠系膜神经发育良好；（２）ＨＤ狭窄段肠管ＰＧＰ９－５ 阳性神经纤维大量增生，神经节细胞缺如。结论：（１）Ｔｒｉｓｏｍｙ １６ 鼠具有稳定的遗传学特征，可能伴先天性巨结肠。（２） 由于ＨＤ 狭窄段肠管神经节细胞缺失，增生的ＰＧＰ９－５ 阳性神经纤维是肠道外源性神经的代偿，对其神经元的性质尚有待确定。（３）ＨＤ有遗传倾向
OBJECTIVE: To study the neuronal expression of trisomy 16 colorectal neoplasia and protein gene product9. 5 (PGP9-5) in the animal model of Down’s syndrome. Methods: Trisomy 16 mouse culture; cytogenetic analysis; Trisomy 16 murine colon and HDPGP9-5 immunohistochemistry. Results: (1) Trisomy16 mouse colon nervous system dysplasia, myenteric plexus growth retardation, submucosal nerve plexus was missing, 5 mm distal ganglion without ganglion, but the mesenteric nerve was well developed; (2) HD stenosis Paraffin sections of PGP9-5-positive nerve fibers proliferated with absence of ganglion cells. Conclusion: (1) Trisomy 16 mice have stable genetic characteristics, which may be associated with Hirschsprung’s disease. (2) Due to the absence of the ganglion ganglion cells in the narrow segment of HD, the proliferating PGP9-5-positive nerve fibers are compensated by the intestinal exogenous nerves. The nature of their neurons remains to be determined. (3) HD has a genetic predisposition