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作者提出一种新的志贺菌菌苗,它是由提纯的2a型福氏志贺菌或宋内志贺菌的脂多糖(LPS)与C群2b型脑膜炎球菌外膜蛋白的蛋白体(prot)疏水复合而成。 LPS是从2a型福氏志贺菌或宋内志贺菌的分离物经热酚提取。碱脱毒的LPS(LP-Sad)经弱碱处理来制备。蛋白体-脂多糖菌苗(prot-LPS或prot-LPSad)的制备是由纯化的LPS或LPSad与C群2b型脑膜炎球菌外膜蛋白按1:1(重量/重量)混合。蛋白体和LPS或LPSad的最终浓度为2mg/ml。 对7～10周龄的BALB/c小鼠进行免
The authors propose a new Shigella vaccine that is composed of purified proteosomes of the LPS of S. bassiana 2a or S. Shigella and the outer membrane proteins of the group C meningococcal group 2b (prot) hydrophobic composite made. LPS is extracted from hot phenol from isolates of Type 2a Shigella flexneri or Shigella sonnei. Base detoxified LPS (LP-Sad) was prepared by weak base treatment. Proteosome-lipopolysaccharide vaccines (prot-LPS or prot-LPSad) were prepared by mixing purified LPS or LPSad with Group C, Group 2b meningococcal outer membrane protein at 1: 1 (w / w). The final concentration of proteosome and LPS or LPSad was 2 mg / ml. 7 to 10 weeks old BALB / c mice were immunized