从天津郊区番茄上获得烟草花叶病毒(TMV)58个分离物,按寄主不同症状合并归6类,从中选出10个代表分离物与10个 TMV 单克隆抗体(MCA)进行ELISA 检测试验,结果从10个分离物中识别出5个抗原决定簇,分为5个抗原型。这与根据寄主反应归并的6类相比,10个分离物中有8个是一致的,2个不一致。说明在本试验中,二者之间多数是一致的,但仍有部分是不一致的。 Fifty-eight tobacco mosaic virus (TMV) isolates were obtained from tomatoes in the suburbs of Tianjin. According to the different host symptoms, six types of TMV were grouped together. Ten representative isolates and ten TMV monoclonal antibodies (MCA) Results Five epitopes were identified from 10 isolates and divided into 5 antigen types. This is in contrast to the 6 categories grouped according to the host reaction, in which 8 out of 10 isolates are identical and 2 are not identical. Explanation In this experiment, most of them are the same, but some of them are inconsistent.