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目的研究CYP1A1 Exon7和GSTM3基因多态性与内蒙古地区汉族肺癌易感性的关系。方法采用等位基因特异性扩增法(ASA)和限制性片断长度多态性(PCR-RFLP)技术对324例汉族非肺部疾病患者和174例汉族肺癌患者进行CYP1A1 Exon7及GSTM3基因多态性分析;同时研究其与吸烟及肺癌之间的相互关系。结果肺癌组与对照组的CYP1A1 Exon7、GSTM3基因多态性差异均无统计学意义(P>0.05);吸烟人群患肺癌的危险性是不吸烟人群的2.107倍(OR=2.107,95%CI=1.44～3.080);携带CYP1A1 Exon7基因突变纯合型(Val/Val)的个体患肺癌风险增高(OR=1.576);携带CYP1A1 Exon7基因突变杂合型和突变纯合型(Ile/Val+Val/Val)并且吸烟的个体患肺癌的风险增高(OR=2.503)。结论吸烟为肺癌的易感因素,CYP1A1 Exon7基因突变杂合型和突变纯合型是肺癌的可疑易感因素,和吸烟在肺癌易感性方面具有协同作用;GSTM3基因多态性与肺癌易感性无关。
Objective To investigate the relationship between CYP1A1 Exon7 and GSTM3 gene polymorphisms and lung cancer susceptibility in Han nationality in Inner Mongolia. Methods CYP1A1 Exon7 and GSTM3 polymorphisms were detected in 324 Han patients with non-pulmonary disease and 174 Han patients with lung cancer using allele-specific amplification (ASA) and restriction fragment length polymorphism (PCR-RFLP) Sex analysis; at the same time to study its relationship with smoking and lung cancer. Results There was no significant difference in the polymorphism of CYP1A1 gene Exon7 and GSTM3 among lung cancer patients and controls (P> 0.05). The risk of lung cancer in smokers was 2.107 times more than that in nonsmokers (OR = 2.107, 95% CI = 1.44 ~ 3.080). The CYP1A1 Exon7 mutation homozygous (Val / Val) individuals had an increased risk of lung cancer (OR = 1.576) Val) and smokers have a higher risk of lung cancer (OR = 2.503). Conclusions Smoking is a predisposing factor for lung cancer. The heterozygous and homozygous mutation of CYP1A1 Exon7 gene is a suspicious predisposing factor for lung cancer and has a synergistic effect with smoking in lung cancer susceptibility. GSTM3 gene polymorphism is not associated with lung cancer susceptibility .