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背景:近年来脂肪源性基质细胞成为研究与应用的热点之一,但前列腺素 E1 对脂肪源性基质细胞增殖的作用影响尚未见报道。目的:观察前列腺素 E1 对人类脂肪源性基质细胞体外增殖的影响。方法:从人正常脂肪组织分离培养脂肪源性基质细胞,用 5 种不同浓度的前列腺素 E1 处理培养的细胞,其中 3×10-6,6×10-6,9×10-6,12×10-6 mol/L 作为实验组,0×10-6 mol/L 作为对照组,观察经不同浓度前列腺素 E1 处理后细胞的增殖情况。结果与结论:不同浓度实验组的细胞数量与对照组相比均显著升高,差异有显著性意义(P<0.05),各实验组内相比,细胞数量增殖无显著性差异(P>0.05)。提示前列腺素 E1 具有促进脂肪源性基质细胞的增殖作用,且前列腺素 E1 的影响效果无剂量依赖性。
BACKGROUND: Adipose-derived stromal cells have become one of the hot topics in recent years. However, the effect of prostaglandin E1 on the proliferation of adipose-derived stromal cells has not been reported yet. Objective: To observe the effect of prostaglandin E1 on the proliferation of human adipose derived stromal cells in vitro. Methods: Adipose-derived stromal cells were isolated and cultured from human normal adipose tissue. Cultured cells were treated with 5 different concentrations of prostaglandin E1, of which 3 × 10-6, 6 × 10-6, 9 × 10-6, 12 × 10-6 mol / L as experimental group and 0 × 10-6 mol / L as control group. The proliferation of cells treated with different concentrations of prostaglandin E1 was observed. RESULTS AND CONCLUSION: Compared with the control group, the number of cells in different concentrations of test group were significantly increased (P <0.05). There was no significant difference in the number of cells in each experimental group (P> 0.05 ). Prompted Prostaglandin E1 has the promotion of proliferation of adipose derived stromal cells, and the effect of prostaglandin E1 no dose-dependent.