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细胞分裂在鲽形目鱼类变态过程中发挥了重要作用。Epigen作为表皮分裂原,通过激活MAPK通道来调控有丝分裂。以前的研究利用定量PCR表明,epigen在变态前期表达量升高,而变态后期表达量下降,表明其与变态发育相关。为了进一步调查epigen与牙鲆变态发育的关系,利用RNA整体原位杂交技术检测了epigen在变态期牙鲆体内的空间表达分布,发现在变态期的各个阶段,epigen基因表达分布均比较广泛,表皮、鳃、肝脏、肠道、鳍条、支鳍骨、肌节等组织都有表达,在牙鲆变态前和变态早期(E期)信号较强,而在变态高峰期(F期和G期)和变态后期(H期)信号逐渐变弱。Epigen空间表达分布模式提示,其作为表皮分裂原,可能广泛参与牙鲆变态发育早期事件,尤其可能与肝脏、肠道、支鳍骨和鳍条的发育有关。
Cell division plays an important role in the metamorphosis of the halibut fish. Epigen, as an epidermal cell precursor, regulates mitosis by activating the MAPK pathway. Previous studies using quantitative PCR showed that epigen increased in pre-metamorphosis and decreased in late metamorphosis, suggesting that epigen is associated with metamorphosis. In order to further investigate the relationship between epigen and Japanese flounder metamorphosis, epigen was detected by RNA in situ hybridization. The distribution of epigen gene was found in all stages of metamorphosis. , Gill, liver, gut, ray, branch fin, sarcolemmal and other tissues were expressed in the pre-metamorphosis and early metamorphosis (E) signal strong, and in the abnormal peak (F and G ) And metamorphosis late (H period) signal gradually weakened. The Epigen spatial expression distribution pattern suggests that it may play an important role in the early stage of metamorphosis of the Japanese flounder, which may play an important role in the development of the liver, intestine, branches and fins.