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目的:观察归芍颗粒(Guishao granules,GG)对四氯化碳(carbon tetrachloride,CCl4)诱导的SD大鼠肝纤维化的作用。方法:90只雄性SD大鼠,除正常组15只不造模外,其余大鼠背部ih CCl4(1 m L·kg-1),每周2次,共13周制备肝纤维化模型,随机分为5组,分别为模型组,GG低、中、高剂量组(3.5,7.0,14 g·kg-1)和秋水仙碱组(0.1 mg·kg-1),每组15只。13周后,测定大鼠肝脏指数;取部分肝组织做苏木素-伊红(HE)和马松三色(Masson)染色;取血测血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的活性;放射免疫法检测血清中透明质酸(HA)和层粘连蛋白(LN)的水平;分光光度法检测大鼠肝脏中羟脯氨酸(Hyp)含量;免疫组化法检测肝脏中α-平滑肌肌动蛋白(α-SMA)的表达水平。结果:与正常组比较,模型组大鼠肝损伤,肝纤维化程度明显,肝纤维化大鼠血清中ALT,AST和HA,LN水平明显升高,肝组织中Hyp的含量明显升高,肝组织中α-SMA蛋白的表达明显升高(P<0.05,P<0.01);GG能明显改善大鼠肝损伤,减轻肝纤维化程度,同时显著降低肝纤维化大鼠血清中ALT,AST和HA,LN水平,GG能降低肝组织中Hyp的含量,抑制肝组织中α-SMA蛋白的表达(P<0.05,P<0.01)。结论:GG可以明显改善CCl4诱导的大鼠肝纤维化,其作用机制可能与GG抑制肝星状细胞活化有关。
Objective: To observe the effects of Guishao granules (GG) on liver fibrosis induced by carbon tetrachloride (CCl4) in SD rats. Methods: Ninety male Sprague-Dawley rats were randomly divided into normal group (n = 15), model group (ih CCl4 (1 m L · kg -1), twice a week for 13 weeks) The rats in each group were divided into 5 groups: model group, GG low, medium and high dose groups (3.5, 7.0 and 14 g · kg -1) and colchicine group (0.1 mg · kg -1), 15 rats in each group. Thirteen weeks later, the liver index of rats was determined. Some liver tissues were stained with hematoxylin and eosin (HE) and Masson staining. Serum levels of alanine aminotransferase (ALT) and aspartate (AST). Radioimmunoassay (RIA) was used to detect the levels of HA and LN. The contents of hydroxyproline (Hyp) in rat liver were detected by spectrophotometry. Histochemistry was used to detect the expression of α-smooth muscle actin (α-SMA) in the liver. Results: Compared with the normal group, the liver injury and hepatic fibrosis in the model group were significant. The levels of ALT, AST, HA and LN in the serum of liver fibrosis rats were significantly increased, and the content of Hyp in the liver tissue was significantly increased. (P <0.05, P <0.01); GG can significantly improve liver injury, reduce the degree of liver fibrosis, and significantly reduce the serum levels of ALT, AST and HA and LN, GG could reduce the content of Hyp in liver tissue and inhibit the expression of α-SMA protein in liver tissue (P <0.05, P <0.01). CONCLUSION: GG can significantly improve CCl4-induced hepatic fibrosis in rats, and its mechanism may be related to the inhibition of hepatic stellate cell activation by GG.