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Gibberellin A4 is effectively separated from mixture of gibberellin A4 and its analogue gibberellin A7,in the form of its crystalline complex with N,N,N,N-tetramethylethylenediamine.After removing the tetramethylethylenediamine from the crystal by dilute HCl,gibberellin A4 is obtained a purity of 98.1% and a yield of 72.7%.