论文部分内容阅读
小檗碱(BBR)是一种天然小分子药物,具有多种药理活性,作用于多个靶点。我们在之前的研究中发现,小檗碱具有稳定mR NA、避免其降解的活性,并初步证实这与它和mR NA 3′端polyA尾部的结合有关,然而其具体机制尚不清楚。本文首次报道了相关的机制研究。研究结果表明,小檗碱结合于poly A、避免mR NA降解的这一过程需要PABP(poly A结合蛋白)参与。利用RNA-EMSA(RNA凝胶电泳迁移)、RIP(RNA免疫共沉淀)和荧光光谱等多项技术证明了小檗碱能够促进PABP与poly A的结合,从而增强mR NA的稳定性。此外利用二维核磁共振技术揭示BBR与AMP结合的特异性。本文结论是小檗碱促进蛋白表达的机制与其作用于poly A、稳定m RNA,从而促进蛋白翻译、上调蛋白表达相关。
Berberine (BBR) is a natural small molecule drug with multiple pharmacological activities that act on multiple targets. In our previous study, we found that berberine has a stable mR NA to prevent its degradation, and preliminary evidence indicates that it is related to its binding to the 3 ’polyA tail of mR NA. However, the exact mechanism remains unclear. This article first reported the related mechanism research. The results show that the process of binding of berberine to poly A to avoid the degradation of mR NA requires the involvement of PABP (poly A binding protein). Several techniques such as RNA-EMSA (RNA gel electrophoresis), RIP (immunoprecipitation) and fluorescence spectroscopy were used to demonstrate that berberine can promote the binding of PABP to poly A and enhance the stability of mR NA. In addition, two-dimensional nuclear magnetic resonance technique was used to reveal the specificity of BBR binding to AMP. The conclusion of this paper is that the mechanism of berberine to promote protein expression and its role in poly A, stable m RNA, thereby promoting protein translation, upregulation of protein expression.