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目的:探讨螺旋藻改善铝中毒小鼠学习记忆障碍的作用机制。方法:用口服氯化铝方法建立小鼠铝中毒学习记忆障碍模型,并用螺旋藻多糖灌胃进行实验性治疗。用跳台试验和避暗试验检测各组小鼠的学习记忆行为,并用免疫组化ABC法检测各组小鼠海马神经元型一氧化氮合酶(nNOS)的表达。结果:与对照组比较,模型组小鼠表现出明显的学习记忆障碍,海马nNOS表达下降。实验组的学习记忆能力较模型组有明显改善,海马nNOS表达增加。结论:螺旋藻对铝中毒小鼠的学习记忆障碍有明显的改善作用,其作用机制可能与增加nNOS的表达有关。
Objective: To explore the mechanism of Spirulina in improving learning and memory impairment in mice with aluminum toxicity. METHODS: The model of learning and memory impairment of aluminum poisoning in mice was established by oral aluminum chloride method, and experimental treatment was performed by intragastric administration of Spirulina polysaccharide. The learning and memory behaviors of mice in each group were detected by step-down test and dark-shelter test, and the expression of neuronal nitric oxide synthase (nNOS) in hippocampus of each group was detected by immunohistochemical ABC method. RESULTS: Compared with the control group, the mice in the model group showed significant impairment in learning and memory, and the expression of nNOS in the hippocampus decreased. The learning and memory ability of the experimental group was significantly improved compared with the model group, and the expression of nNOS in the hippocampus was increased. Conclusion: Spirulina can significantly improve the learning and memory impairment in aluminum poisoning mice. The mechanism may be related to the increase of nNOS expression.