论文部分内容阅读
目的建立人CTRP4基因的转基因小鼠,为脂肪细胞因子CTRP4的体内功能研究奠定基础。方法首先构建人CTRP4的转基因小鼠线性化表达载体,再利用显微注射的方法将载体注射入小鼠受精卵,从而构建人CTRP4的首建鼠(Founder)并与野生型小鼠交配繁殖得到F1代阳性小鼠,再通过近亲繁殖与测交的方法,得到CTRP4转基因纯合子小鼠,并通过PCR和western blot的方法对纯合子小鼠进行鉴定。结果得到人CTRP4转基因小鼠纯合子小鼠两个品系,western blot鉴定该转基因小鼠心脏,肝脏,脑,肾脏等多种组织中均呈现CTRP4高表达。结论成功构建了人CTRP4转基因小鼠纯合子小鼠。
Objective To establish transgenic mice with human CTRP4 gene and lay the foundation for the in vivo function of adipocyte cytokine CTRP4. Methods The linearized expression vector of human CTRP4 was constructed and injected into mouse fertilized eggs by microinjection to construct the founder of human CTRP4 and to breed with wild-type mice F1 generation of positive mice, and then by inbreeding and cross dating method to obtain CTRP4 transgenic homozygous mice, and by homozygous mice and PCR and western blot method to identify. Results Two lines of homozygous mice were obtained from human CTRP4 transgenic mice. Western blot analysis showed that CTRP4 was highly expressed in various tissues such as heart, liver, brain and kidney in this transgenic mouse. Conclusion Human CTRP4 transgenic mouse homozygous mice were successfully constructed.