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采用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)研究了序批式活性污泥法(SBR)处理红霉素生产废水过程中从厌氧处理阶段到好氧处理阶段的微生物群落结构变化,并对微生物群落的部分优势细菌进行了克隆测序和系统发育树分析。结果表明,微生物群落丰富度随SBR处理进程呈递增趋势;微生物群落的相似性在相邻的处理阶段间相对较高,相隔越远的处理阶段间则越低;6种优势菌的同源性均在95%以上,其中有4种细菌均为厌氧和好氧处理阶段的优势菌,有1种细菌仅为好氧处理阶段的优势菌。
Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to study the microorganisms from the anaerobic treatment stage to the aerobic treatment stage in the treatment of erythromycin production wastewater by sequencing batch activated sludge process (SBR) Community structure changes, and some dominant bacteria of the microbial community were cloned and sequenced and phylogenetic tree analysis. The results showed that the microbial community abundance increased with the progress of SBR process. The similarity of microbial communities was relatively high between adjacent treatment stages and lower between treatment stages. The homology of the six dominant bacteria All above 95%. Among them, four kinds of bacteria were the dominant bacteria in the stage of anaerobic and aerobic treatment. One kind of bacteria was only the predominant bacteria in aerobic stage.