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目的:观察活性维生素D3腹腔注射对2型糖尿病大鼠海马神经元的影响。方法:SD大鼠30只,随机分为空白对照组(Control),高脂组(HF),高脂+VD组(HF+VD),糖尿病组(DM),糖尿病+VD组(DM+VD)。测定大鼠空腹血糖、胰岛素、维生素D等生化指标,应用透射电镜观察海马神经元结构变化。结果:与对照组相比,HF组、HF+VD组大鼠体重增加,(分别为343.28±10.41 g;356.81±36.20 g和360.18±25.56g),DM组体重降低(265.6±16.11 g)(P<0.05);DM组SDF-1为398.33±15.01 ng/L,低于Control组(457.53±26.56 ng/L),骨化三醇干预后SDF-1显著升高(448.54±36.83 ng/L)(P<0.05);DM组(25-OH-D)水平为165.25±30.75μg/L,低于Control组213.65±30.79μg/L,补充骨化三醇后显著提高至210.31±50.69μg/L(P<0.05);DM组海马神经元CXCR4表达降低,线粒体可见明显肿胀,DM+VD组线粒体形态明显改善,海马神经元CXCR4表达水平明显升高。结论:常规量补充维生素D可提高糖尿病大鼠体内维生素D水平,对海马神经元有明显保护作用。
Objective: To observe the effect of intraperitoneal injection of active vitamin D3 on hippocampal neurons in type 2 diabetic rats. Methods: Thirty SD rats were randomly divided into control group, HF group, HF + VD group, DM group, DM + VD group ). The fasting blood glucose, insulin, vitamin D and other biochemical markers were determined. The changes of hippocampal neurons were observed by transmission electron microscopy. Results: Compared with the control group, the body weight of HF group and HF + VD group increased (343.28 ± 10.41 g, 356.81 ± 36.20 g and 360.18 ± 25.56 g, respectively), and the body weight of DM group decreased (265.6 ± 16.11 g) SDF-1 was 398.33 ± 15.01 ng / L in DM group, which was lower than that in Control group (457.53 ± 26.56 ng / L). SDF-1 was significantly increased after intervention with calcitriol (448.54 ± 36.83 ng / L (P <0.05). The level of 25-OH-D in DM group was 165.25 ± 30.75μg / L, which was lower than 213.65 ± 30.79μg / L in Control group, and significantly increased to 210.31 ± 50.69μg / (P <0.05). CXCR4 expression in hippocampal neurons was decreased in DM group and mitochondria were significantly swollen. The mitochondria morphology in DM + VD group was significantly improved, and CXCR4 expression in hippocampal neurons increased significantly. CONCLUSION: Conventional vitamin D supplementation can increase the level of vitamin D in diabetic rats and significantly protect the hippocampal neurons.