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目的研究毛重楼根茎中的化学成分。方法采用溶剂法提取,利用正相硅胶色谱、葡聚糖凝胶SephadexLH-20、反相制备色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR等波谱技术进行结构鉴定。结果从醋酸乙酯层和正丁醇层中分离得到11个化合物,分别鉴定为β-谷甾醇(Ⅰ)、豆甾醇(Ⅱ)、邻苯二酸二正丁基酯(Ⅲ)、β-蜕皮激素(Ⅳ)、偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅴ)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅵ)、偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-β-D-吡喃葡萄糖苷(Ⅶ)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-β-D-吡喃葡萄糖苷(Ⅷ)、偏诺皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅸ)、槲皮素(Ⅹ)和山柰酚(Ⅺ)。结论化合物Ⅰ～Ⅺ均为首次从该种植物中分离得到,其中化合物Ⅲ、Ⅹ为首次从该属植物中分离得到。
Objective To study the chemical constituents in the rhizomes of the barnyardgrass. Methods The solvent extraction method was used to separate and purify the compounds by normal phase silica gel chromatography, Sephadex LH-20 and reversed-phase preparative chromatography. The structure was identified by 1H-NMR and 13C-NMR spectroscopy. Results Eleven compounds were isolated from the ethyl acetate layer and the n-butanol layer and identified as β-sitosterol (I), stigmasterol (II), di-n-butyl phthalate (III), and β-carotene respectively. Hormone (IV), Parthenosaponin-3-O-α-L-arabinofuranosyl (1→4)-[α-L-rhamnopyranosyl (1→2)]-β-D- Glucopyranoside (V), diosgenin-3-O-α-L-arabinofuranosyl (1→4)-[α-L-rhamnopyranosyl (1→2)]-β- D-glucopyranoside (VI), trimethoprim-3-O-α-L-arabinofuranosyl (1→4)-β-D-glucopyranoside (VII), diosgenin-3 -O-α-L-arabinofuranosyl (1→4)-β-D-glucopyranoside (VIII), 3-n-saponin-3-O-α-L-rhamnopyranosyl (1) →4)-α-L-rhamnopyranosyl (1→4)-[α-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside (IX), Quercetin (X) and Kaempfol (XI). Conclusion Compounds I-XI were isolated from this plant for the first time. Compounds III and X were isolated from this plant for the first time.