目的:观察负压封闭引流技术(VSD)对创面细菌数、G-/G+比例及创面细菌种属数量的变化,探讨负压封闭引流技术控制创面感染的机制。方法:12只实验兔创面模型建立后,随机分为负压治疗组和常规换药组。于治疗前后各时间点取创面活组织,匀浆后在琼脂糖平板上培养,然后进行细菌计数、细菌种属分类计数。革兰氏染色,计算革兰氏阴性(G-)菌的比例,并观察感染和创面愈合情况。结果:与常规换药组相比,负压组创面细菌数明显低于常规换药组(P<0.05)并维持在较低水平;G-细菌的比例明显降低(P<0.05)。负压组创面葡萄球菌属、杆菌属的数量显著减少(P<0.05),对创面中链球菌属及其他菌属数量的改变也有一定的作用,与比常规换药组相比差异无统计学意义(P>0.05)。结论:负压封闭引流可以有效促进兔软组织创面内细菌清除,更好地控制创面感染形成,促进创面愈合。 OBJECTIVE: To observe the change of bacterial count, G- / G + ratio and the number of bacterial species in the wounds with negative pressure closed drainage (VSD) technique and to explore the mechanism of negative pressure closed drainage to control wound infection. Methods: After establishment of 12 experimental rabbits, they were randomly divided into negative pressure treatment group and conventional dressing group. Before and after treatment, wound tissue was taken at each time point, homogenized and then cultured on agar plates. Bacterial count and bacterial species were counted. Gram stain, calculate the proportion of Gram-negative (G-) bacteria, and observe the infection and wound healing. Results: Compared with the conventional dressing group, the number of bacteria in the negative pressure group was significantly lower than that of the conventional dressing group (P <0.05) and maintained at a low level; the proportion of G-bacteria was significantly decreased (P <0.05). Negative pressure group wound Staphylococcus, Bacillus were significantly reduced (P <0.05), the wound surface of Streptococcus and other bacteria also have a certain amount of change, compared with the conventional dressing group, the difference was not statistically significant Significance (P> 0.05). Conclusion: Closed-circuit drainage with negative pressure can effectively promote bacterial clearance in rabbit soft tissue wounds and better control the wound infection and promote wound healing.